Wnt3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18245
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide from the C-terminal of Human Proto-oncogene Wnt-3 (aa. 280-330)
Localization
Secreted|Extracellular Space|Extracellular Matrix
Specificity
50 kDa band is due to post translational modifications.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Wnt3 Antibody
Western Blot: Wnt3 Antibody [NBP3-18245]
Western Blot: Wnt3 Antibody [NBP3-18245] - Western blot analysis of Mouse Brain showing detection of 39.6 kDa Wnt3 protein using Rabbit Anti-Wnt3 Polyclonal Antibody (NBP3-18245). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Wnt3 Polyclonal Antibody (NBP3-18245) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 39.6 kDa. Other Band(s): 50 kDa.Immunohistochemistry: Wnt3 Antibody [NBP3-18245]
Immunohistochemistry: Wnt3 Antibody [NBP3-18245] - Immunohistochemistry analysis using Rabbit Anti-Wnt3 Polyclonal Antibody (NBP3-18245). Tissue: Breast. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-Wnt3 Polyclonal Antibody (NBP3-18245) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.Applications for Wnt3 Antibody
Application
Recommended Usage
Immunohistochemistry
1:50
Western Blot
1:1000
Application Notes
Optimal dilutions for assays should be determined by the user.
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS pH 7.4, 50% glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Wnt-3
Long Name
Wingless-type MMTV Integration Site Family, Member 3
Alternate Names
INT4, TETAMS, WNT3
Gene Symbol
WNT3
Additional Wnt-3 Products
Product Documents for Wnt3 Antibody
Product Specific Notices for Wnt3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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