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WSTF Antibody (5E9)

Novus Biologicals, part of Bio-Techne | Catalog # H00009031-M01

Novus Biologicals, part of Bio-Techne
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H00009031-M01-100ug

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Proximity Ligation Assay, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 5E9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

BAZ1B (NP_075381, 1384 a.a. ~ 1483 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDFQTVQNKCSCGSYRSVQEFLTDMKQVFTNAEVYNCRGSHVLSCMVKTEQCLVALLHKHLPGHPYVRRKRKKFPDRLAEDEGDSEPEAVGQSRGRRQKK

Localization

Nuclear

Specificity

BAZ1B - bromodomain adjacent to zinc finger domain, 1B

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for WSTF Antibody (5E9)

Immunohistochemistry-Paraffin: WSTF Antibody (5E9) [H00009031-M01]

Immunohistochemistry-Paraffin: WSTF Antibody (5E9) [H00009031-M01]

Immunohistochemistry-Paraffin: WSTF Antibody (5E9) [H00009031-M01] - Analysis of monoclonal antibody to BAZ1B on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml.
Proximity Ligation Assay: WSTF Antibody (5E9) [H00009031-M01]

Proximity Ligation Assay: WSTF Antibody (5E9) [H00009031-M01]

Proximity Ligation Assay: WSTF Antibody (5E9) [H00009031-M01] - Analysis of protein-protein interactions between SMARCB1 and BAZ1B. HeLa cells were stained with anti-SMARCB1 rabbit purified polyclonal 1:1200 and anti-BAZ1B mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Proximity Ligation Assay: WSTF Antibody (5E9) [H00009031-M01]

Proximity Ligation Assay: WSTF Antibody (5E9) [H00009031-M01]

Proximity Ligation Assay: WSTF Antibody (5E9) [H00009031-M01] - Analysis of protein-protein interactions between SMARCB1 and BAZ1B. Huh7 cells were stained with anti-SMARCB1 rabbit purified polyclonal 1:1200 and anti-BAZ1B mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Applications for WSTF Antibody (5E9)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Proximity Ligation Assay

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recombinant protein on ELISA. It has been used for IHC-P.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: WSTF

WSTF (Williams syndrome transcription factor) is a member of the bromodomain protein family. The bromodomain is a structural motif characteristic of proteins involved in chromatin-dependent regulation of transcription. WSTF forms a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. This gene is deleted in Williams-Beuren syndrome, a developmental disorder.

Alternate Names

bromodomain adjacent to zinc finger domain, 1B, williams-Beuren syndrome chromosomal region 10 protein

Entrez Gene IDs

9031 (Human)

Gene Symbol

BAZ1B

OMIM

605681 (Human)

Additional WSTF Products

Product Documents for WSTF Antibody (5E9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for WSTF Antibody (5E9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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