WT1 Antibody (SPM361) - IHC-Prediluted
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48215
![Immunohistochemistry-Paraffin: WT1 Antibody (SPM361) - IHC-Prediluted [NBP2-48215]](https://resources.bio-techne.com/images/products/WT1-Antibody-SPM361-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48215-img0001.jpg "Immunohistochemistry-Paraffin: WT1 Antibody (SPM361) - IHC-Prediluted [NBP2-48215]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM361
Format
IHC-Prediluted
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein corresponding to residues 1-181 of human WT1. (Uniprot: P19544)
Localization
Nuclear and Cytoplasmic
Specificity
Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilms tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.
Marker
Wilm's Tumor & Mesothelial Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
Scientific Data Images for WT1 Antibody (SPM361) - IHC-Prediluted
Immunohistochemistry-Paraffin: WT1 Antibody (SPM361) - IHC-Prediluted [NBP2-48215]
Immunohistochemistry-Paraffin: WT1 Antibody (SPM361) - IHC-Prediluted [NBP2-48215] - Formalin-fixed, paraffin-embedded human Mesothelioma stained with Wilm's TumorMonoclonal Antibody (SPM361).Applications for WT1 Antibody (SPM361) - IHC-Prediluted
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Hu-chromosome location: 11p13
Molecular weight of antigen: 47-55kDa
Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Molecular weight of antigen: 47-55kDa
Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Format
IHC-Prediluted
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: WT1
Defects in the WT1 protein can lead to Wilms tumor, Frasier syndrome, Denys-Drash syndrome, nephrotic syndrome type 4, and Meacham syndrome.
Long Name
Wilms Tumor 1
Alternate Names
GUD, WAGR, WIT-2, WT33
Gene Symbol
WT1
Additional WT1 Products
Product Documents for WT1 Antibody (SPM361) - IHC-Prediluted
Product Specific Notices for WT1 Antibody (SPM361) - IHC-Prediluted
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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