Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI12G7

Concentration

1 mg/ml

View all WTAP Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 2-106 of human WTAP(NP_004897) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

44.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for WTAP Antibody (OTI12G7)

Western Blot: WTAP Antibody (OTI12G7) [NBP2-46368]

Western Blot: WTAP Antibody (OTI12G7) [NBP2-46368]

Western Blot: WTAP Antibody (OTI12G7) [NBP2-46368] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WTAP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiWTAP.(1:2000)
Immunohistochemistry-Paraffin: WTAP Antibody (OTI12G7) [NBP2-46368]

Immunohistochemistry-Paraffin: WTAP Antibody (OTI12G7) [NBP2-46368]

Immunohistochemistry-Paraffin: WTAP Antibody (OTI12G7) [NBP2-46368] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-WTAP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150)
Immunohistochemistry-Paraffin: WTAP Antibody (OTI12G7) [NBP2-46368]

Immunohistochemistry-Paraffin: WTAP Antibody (OTI12G7) [NBP2-46368]

Immunohistochemistry-Paraffin: WTAP Antibody (OTI12G7) [NBP2-46368] - Staining of paraffin-embedded Human tonsil using anti-WTAP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,) (1:150)
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Applications for WTAP Antibody (OTI12G7)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: WTAP

The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in three transcript variants, two of which encode the same isoform. [provided by RefSeq]

Alternate Names

Female-lethal(2)D homolog, hFL(2)D, KIAA0105DKFZp686F20131, MGC3925, PNAS-132, pre-mRNA-splicing regulator WTAP, putative pre-mRNA splicing regulator female-lethal(2D), Wilms tumor 1 associated protein, Wilms tumor 1-associating protein, Wilms' tumour 1-associating protein, WT1-associated protein

Gene Symbol

WTAP

Additional WTAP Products

Product Documents for WTAP Antibody (OTI12G7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for WTAP Antibody (OTI12G7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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