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WTAP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31777

Novus Biologicals, part of Bio-Techne
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NBP1-31777

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human WTAP. The exact sequence is proprietary.

Localization

Nucleus, nucleolus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WTAP Antibody

Western Blot: WTAP Antibody [NBP1-31777]

Western Blot: WTAP Antibody [NBP1-31777]

Western Blot: WTAP Antibody [NBP1-31777] - Jurkat whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with WTAP antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: WTAP Antibody [NBP1-31777]

Immunocytochemistry/ Immunofluorescence: WTAP Antibody [NBP1-31777]

Immunocytochemistry/Immunofluorescence: WTAP Antibody [NBP1-31777] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution. Alpha-tubulin filaments were labeled with Red at 1:2000.
Immunohistochemistry: WTAP Antibody [NBP1-31777]

Immunohistochemistry: WTAP Antibody [NBP1-31777]

Immunohistochemistry: WTAP Antibody [NBP1-31777] - Analysis of paraffin-embedded A549 Xenograft, using WTAP antibody at 1:100 dilution.

Applications for WTAP Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: WTAP

The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in three transcript variants, two of which encode the same isoform.

Alternate Names

Female-lethal(2)D homolog, hFL(2)D, KIAA0105DKFZp686F20131, MGC3925, PNAS-132, pre-mRNA-splicing regulator WTAP, putative pre-mRNA splicing regulator female-lethal(2D), Wilms tumor 1 associated protein, Wilms tumor 1-associating protein, Wilms' tumour 1-associating protein, WT1-associated protein

Gene Symbol

WTAP

Additional WTAP Products

Product Documents for WTAP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for WTAP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.