WTAP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83040

Novus Biologicals, part of Bio-Techne
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NBP1-83040
NBP1-83040-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all WTAP Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DEEVEGMQSTILVLQQQLKETRQQLAQYQQQQSQASAPSTSRTTASEPVEQSEATSKDCSRLTNGPSNGSSSRQRTSGSGFHREGNTTEDDFPSSPGNGNKSSNSSEERTGRGGSGYVNQLSAGYESVDSPTGSENSLTHQSND

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WTAP Antibody

Western Blot: WTAP Antibody [NBP1-83040]

Western Blot: WTAP Antibody [NBP1-83040]

Western Blot: WTAP Antibody [NBP1-83040] - Analysis in control (vector only transfected HEK293T lysate) and WTAP over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: WTAP Antibody [NBP1-83040]

Immunocytochemistry/ Immunofluorescence: WTAP Antibody [NBP1-83040]

Immunocytochemistry/Immunofluorescence: WTAP Antibody [NBP1-83040] - Staining of human cell line U-2 OS shows localization to nuclear speckles. Antibody shown in green.
Immunohistochemistry-Paraffin: WTAP Antibody [NBP1-83040]

Immunohistochemistry-Paraffin: WTAP Antibody [NBP1-83040]

Immunohistochemistry-Paraffin: WTAP Antibody [NBP1-83040] - Staining of human prostate shows moderate to strong nuclear positivity.
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Applications for WTAP Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: WTAP

The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in three transcript variants, two of which encode the same isoform. [provided by RefSeq]

Alternate Names

Female-lethal(2)D homolog, hFL(2)D, KIAA0105DKFZp686F20131, MGC3925, PNAS-132, pre-mRNA-splicing regulator WTAP, putative pre-mRNA splicing regulator female-lethal(2D), Wilms tumor 1 associated protein, Wilms tumor 1-associating protein, Wilms' tumour 1-associating protein, WT1-associated protein

Gene Symbol

WTAP

Additional WTAP Products

Product Documents for WTAP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for WTAP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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