Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all WWP2 Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 200 and 250 of human WW Domain Containing E3 Ubiquitin Protein Ligase 2 using the numbering given in entry NP_008945.2 (GeneID 11060).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WWP2 Antibody - BSA Free

Western Blot: WWP2 Antibody [NBP1-49942]

Western Blot: WWP2 Antibody [NBP1-49942]

Western Blot: WWP2 Antibody [NBP1-49942] - Detection of mouse WWP2 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-WWP2 antibody NBP1-49942 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: WWP2 Antibody [NBP1-49942]

Western Blot: WWP2 Antibody [NBP1-49942]

Western Blot: WWP2 Antibody [NBP1-49942] - Detection of human WWP2 by western blot. Samples: Whole cell lysate from HEK293T (5, 15 and 50 ug), and HeLa (50 ug), and Jurkat (50 ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-WWP2 antibody NBP1-49942 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: WWP2 Antibody [NBP1-49942]

Immunoprecipitation: WWP2 Antibody [NBP1-49942]

Immunoprecipitation: WWP2 Antibody [NBP1-49942] - Detection of human WWP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-WWP2 antibody NBP1-49942 used for IP at 6 ug per reaction. WWP2 was also immunoprecipitated by another rabbit anti-WWP2 antibody. For blotting immunoprecipitated WWP2, NBP1-49942 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for WWP2 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: WWP2

This gene encodes a member of the NEDD4-like protein family. The family of proteins is known to possess ubiquitin-protein ligase activity. The encoded protein contains 4 tandem WW domains. The WW domain is a protein motif consisting of 35 to 40 amino acids and is characterized by 4 conserved aromatic residues. The WW domain may mediate specific protein-protein interactions. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Alternate Names

AIP2Nedd-4-like ubiquitin-protein ligase, atrophin-1 interacting protein 2, Atrophin-1-interacting protein 2, EC 6.3.2, EC 6.3.2.-, NEDD4-like E3 ubiquitin-protein ligase WWP2, WW domain containing E3 ubiquitin protein ligase 2, WW domain-containing protein 2, WWp2-like

Gene Symbol

WWP2

Additional WWP2 Products

Product Documents for WWP2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for WWP2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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