Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all XAB1 Antibodies »

Product Specifications

Immunogen

This antibody maps to a region between residue 324 to 374 of human GPN-Loop GTPase 1 using the numbering given in entry BAB17612.1 (GeneID 11321).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for XAB1 Antibody - BSA Free

Western Blot: XAB1 Antibody [NBP2-37686]

Western Blot: XAB1 Antibody [NBP2-37686]

Western Blot: XAB1 Antibody [NBP2-37686] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Affinity purified rabbit anti-GPN1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: XAB1 Antibody [NBP2-37686]

Immunoprecipitation: XAB1 Antibody [NBP2-37686]

Immunoprecipitation: XAB1 Antibody [NBP2-37686] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Affinity purified rabbit anti-GPN1 antibody used for IP at 6 ug per reaction. GPN1 was also immunoprecipitated by rabbit anti-GPN1 antibody. For blotting immunoprecipitated GPN1, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for XAB1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: XAB1

The DNA methylation system is composed of methyl-CpG-binding proteins, as well as of DNA cytosine methyl transferases. Five methyl-CpG binding proteins were isolated: MeCP2, MBD1, MBD2, MBD3 and MBD4. MBD2 consists of two isoforms, MBD2a and MBD2b, which are generated from a single gene. MBD2a is a component of MeCP1, a large corepressor complex that represses transcription from densely methylated genes. Components of MeCP1 include MBD2, Mi-2, MTA2, MBD3 and HDAC1/2. MIZF and MBDin were isolated as proteins interacting with MBD2. MBDin, in turn, is identical to XPA binding protein 1 (XAB1). At the N erminus, the protein contains the consensus sequence for a GTP binding site. At the C terminus, the protein is characterized by an acidic domain containing a cluster of acidic amino acid residues. The transcriptional repression by MBD2 at methylated promoters is relieved by MBdin. XAB1 is expressed ubiquitously, and may be involved in nuclear localization of XPA.

Alternate Names

ATP(GTP)-binding protein, ATPBD1A, GPN-loop GTPase 1, GTPase, MBD2-interacting protein, MBDin, MBDINXPA binding protein 1, XAB1FLJ51176, XPA-binding protein 1

Gene Symbol

GPN1

UniProt

BAB17612.1 (Human)

Additional XAB1 Products

Product Documents for XAB1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for XAB1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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