Xanthine Oxidase Antibody [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2009
Key Product Details
Species Reactivity
Bovine
Applications
Dot Blot, ELISA, Immunoprecipitation, Western Blot
Label
Biotin
Antibody Source
Polyclonal Rabbit IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Xanthine Oxidase [Bovine Buttermilk] (Uniprot: P80457)
Reactivity Notes
Cross reactivity against Xanthine Oxidase from other sources is unknown.
Localization
Cytoplasmic; Peroxisomal
Specificity
Cross reactivity against Xanthine Oxidase from other sources is unknown.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Xanthine Oxidase [Bovine Buttermilk]
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Xanthine Oxidase [Bovine Buttermilk]
Scientific Data Images for Xanthine Oxidase Antibody [Biotin]
Dot Blot: Xanthine Oxidase Antibody [Biotin] [NB100-2009] - Dot Blot results of Rabbit Xanthine Oxidase antibody [Biotin].Dots are Xanthine Oxidase at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: 30 min at RT.Primary Antibody: Rabbit Anti-Xanthine Oxidase Antibody Biotin at 1ug/mL for 1hr at RT.Secondary Antibody: Streptavidin-HRP at 1:40,000 for 30min at RT.
![Xanthine Oxidase Antibody [Biotin]](https://resources.bio-techne.com/images/products/nb100-2009_rabbit-polyclonal-xanthine-oxidase-antibody-biotin-23520231750165.jpg "Xanthine Oxidase Antibody [Biotin]")
Xanthine Oxidase Antibody [Biotin]
Dot Blot results of Rabbit Xanthine Oxidase Antibody [Biotin]. Dots are Xanthine Oxidase at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. blocking for 30 min at RT. Primary Antibody: Rabbit Anti-Xanthine Oxidase Antibody Biotin at 1ug/mL for 1hr at RT. Secondary Antibody: Streptavidin-HRPApplications for Xanthine Oxidase Antibody [Biotin]
Application
Recommended Usage
ELISA
1:5000-1:20000
Immunoprecipitation
1:100
Western Blot
1:500-1:5000
Application Notes
This product has been tested by dot blot and is suitable to be assayed against 1.0 ug of Xanthine Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Xanthine Oxidase
Long Name
Xanthine dehydrogenase/oxidase [Includes: Xanthine dehydrogenase
Alternate Names
EC 1.17.3.2, XD, XDH, XDHA, XOR)
Gene Symbol
XDH
UniProt
Additional Xanthine Oxidase Products
Product Documents for Xanthine Oxidase Antibody [Biotin]
Product Specific Notices for Xanthine Oxidase Antibody [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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