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Xanthine Oxidase Antibody [HRP]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2025

Novus Biologicals, part of Bio-Techne
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NB100-2025-0.1mg

Key Product Details

Species Reactivity

Bovine

Applications

Dot Blot, ELISA, Immunoprecipitation, Western Blot

Label

HRP

Antibody Source

Polyclonal Rabbit IgG

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Xanthine Oxidase [Bovine Buttermilk] (Uniprot: P80457)

Reactivity Notes

Cross reactivity against Xanthine Oxidase from other tissues and species may occur but have not been specifically determined

Localization

Cytoplasmic; Peroxisomal

Specificity

Cross reactivity against Xanthine Oxidase from other tissues and species may occur but have not been specifically determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Xanthine Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Xanthine Oxidase [Bovine Buttermilk]

Scientific Data Images for Xanthine Oxidase Antibody [HRP]

Dot Blot: Xanthine Oxidase Antibody [HRP] [NB100-2025] - Dot Blot of Rabbit Xanthine Oxidase antibody [HRP]. Xanthine Oxidase (1) 100ng, (2) 33.33ng, (3) 11.11ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: Anti-Xanthine Oxidase HRP at 1.0ug/mL for 1hr at RT. Secondary Antibody: none.Blocking Buffer for 30mins at RT. Exposure: 0.1sec.
Xanthine Oxidase Antibody [HRP]

Xanthine Oxidase Antibody [HRP]

Dot Blot of Xanthine Oxidase Antibody [HRP]. Xanthine Oxidase (1) 100ng, (2) 33.33ng, (3) 11.11ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: Anti-Xanthine Oxidase HRP at 1.0ug/mL for 1hr at RT. Secondary Antibody: none. Blocking Buffer: BlockOut Buffer

Applications for Xanthine Oxidase Antibody [HRP]

Application
Recommended Usage

ELISA

1:5000-1:20000

Immunoprecipitation

1:100

Western Blot

1:500-1:5000
Application Notes
This product has been tested by dot blot and is suitable to be assayed against 1.0 ug of Xanthine Oxidase [Bovine Buttermilk] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:35,000 to 1:175,000 of the reconstitution concentration is suggested for this product.

NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Gentamicin Sulfate

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Background: Xanthine Oxidase

Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The enzyme is a homodimer. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism. (provided by RefSeq)

Long Name

Xanthine dehydrogenase/oxidase [Includes: Xanthine dehydrogenase

Alternate Names

EC 1.17.3.2, XD, XDH, XDHA, XOR)

Gene Symbol

XDH

UniProt

Additional Xanthine Oxidase Products

Product Documents for Xanthine Oxidase Antibody [HRP]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Xanthine Oxidase Antibody [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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