XBP1 Antibody (3H1G4) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-75514

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3H1G4

Format

BSA Free

Concentration

1 mg/ml

View all XBP1 Antibodies »

Product Specifications

Immunogen

Antibody was raised against recombinant protein corresponding to amino acids 2 to 160 of human XBP-1.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for XBP1 Antibody (3H1G4) - BSA Free

Western Blot: XBP1 Antibody (3H1G4)BSA Free [NBP1-75514]

Western Blot: XBP1 Antibody (3H1G4)BSA Free [NBP1-75514]

Western Blot: XBP1 Antibody (3H1G4) [NBP1-75514] - analysis of 100 ng of XBP-1 recombinant protein with XBP-1 antibody at 1 ug/mL.
XBP1 Antibody (3H1G4) - BSA Free

Immunocytochemistry/ Immunofluorescence: XBP1 Antibody (3H1G4) - BSA Free [NBP1-75514] -

Immunocytochemistry/ Immunofluorescence: XBP1 Antibody (3H1G4) - BSA Free [NBP1-75514] - Immunofluorescence of XBP1 in HepG2 cells with XBP1 antibody at 4 u/mL.
XBP1 Antibody (3H1G4) - BSA Free

Immunocytochemistry/ Immunofluorescence: XBP1 Antibody (3H1G4) - BSA Free [NBP1-75514] -

Immunocytochemistry/ Immunofluorescence: XBP1 Antibody (3H1G4) - BSA Free [NBP1-75514] - Immunocytochemistry of XBP1 in HepG2 cells with XBP1 antibody at 2 u/mL.

Applications for XBP1 Antibody (3H1G4) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

2-4 ug/mL

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 degrees C. Avoid freeze/thaw cycles.

Background: XBP1

X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER). Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94.

Long Name

X-box Binding Protein 1

Alternate Names

HTF, TREB5, XBP2

Gene Symbol

XBP1

UniProt

BAB82982 (Human)

Additional XBP1 Products

Product Documents for XBP1 Antibody (3H1G4) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for XBP1 Antibody (3H1G4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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