Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (100%), Mouse (100%), Primate (100%), Rat (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all XPB Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 575 and 625 of human excision repair cross-complementing rodent repair deficiency, complementation group 3 using the numbering given in entry NP_000113.1 (GeneID 2071).

Reactivity Notes

Orangutan (100%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for XPB Antibody - BSA Free

Immunohistochemistry-Paraffin: XPB Antibody [NB100-61059]

Immunohistochemistry-Paraffin: XPB Antibody [NB100-61059]

Immunohistochemistry-Paraffin: XPB Antibody [NB100-61059] - Detection of human ERCC3 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ERCC3 (NB100-61059). Detection: DAB
Immunoprecipitation: XPB Antibody [NB100-61059]

Immunoprecipitation: XPB Antibody [NB100-61059]

Immunoprecipitation: XPB Antibody [NB100-61059] - Detection of human ERRC3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ERRC3 antibody NB100-61059 used for IP at 3 ug per reaction. ERRC3 was also immunoprecipitated by rabbit anti-ERRC3 antibody NB100-61060. For blotting immunoprecipitated ERRC3, NB100-61060 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for XPB Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:2000 - 1:10000

Immunohistochemistry-Paraffin

1:2000 - 1:10000

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: XPB

ERCC3 is a component of the core TFIIH basal transcription factor and functions as an ATP-dependent 3'-5' DNA helicase. ERCC3 is involved in nucleotide excision repair and is the cause of xeroderma pigmentosum complementation group B (XPB), also known as xeroderma pigmentosum II (XP2). Additionally, it has been found to be the cuase of Cockayne syndrome, and trichothiodystrophy (TTD). XPB is an autosomal recessive disease characterized by skin photosensitivity and a predisposition to skin cancer, and neurological abnormalities. Cockayne syndrome and TTD are similarly characterized by photosensitivity and neural abnormalities.

Long Name

Xeroderma Pigmentosum B

Alternate Names

ERCC3, GTF2H, RAD25, TFIIH

Entrez Gene IDs

2071 (Human)

Gene Symbol

ERCC3

UniProt

NP_000113.1 (Human)

Additional XPB Products

Product Documents for XPB Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for XPB Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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