XRCC3 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-165
![Western Blot: XRCC3 AntibodyBSA Free [NB100-165]](https://resources.bio-techne.com/images/products/XRCC3-Antibody-Western-Blot-NB100-165-img0005.jpg "Western Blot: XRCC3 AntibodyBSA Free [NB100-165]")
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Drosophila, Hamster
Cited:
Human, Hamster, Insect - Drosophila
Applications
Validated:
ICC/IF (Negative), Immunoprecipitation, Western Blot
Cited:
Block/Neutralize, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
A synthetic partial peptide of human XRCC3. [UniProt# O43542]
Reactivity Notes
Drosophila reactivity reported in scientific literature (PMID: 28743001). Hamster reactivity reported in scientific literature (PMID: 24116071).
Localization
Nuclear
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for XRCC3 Antibody - BSA Free
Western Blot: XRCC3 AntibodyBSA Free [NB100-165]
Western Blot: XRCC3 Antibody [NB100-165] - (A) Human XRCC3 (hXRCC3) expression was down regulated by siRNA in human transofrmed fibroblasts. (B) XRCC3 was inactivated in human colon cancer cells by gene targeting [Yoshihara et all., 2004, EMBO J., 23 (3), 670-680]. hXRCC3 was detected by WB using XRCC3 antibody (NB100-165). The star indicates non-specific cross-reactivity of the antibody. Image provided by confirmed customer review.![Western Blot: XRCC3 AntibodyBSA Free [NB100-165]](https://resources.bio-techne.com/images/products/XRCC3-Antibody-Western-Blot-NB100-165-img0004.jpg "Western Blot: XRCC3 AntibodyBSA Free [NB100-165]")
Western Blot: XRCC3 AntibodyBSA Free [NB100-165]
Western Blot: XRCC3 Antibody [NB100-165] - Detection of XRCC3 (38 kDa) from HeLa cell extract using NB100-165 (1:2500). Lanes 1 and 2 contain HTC116 and Hela whole cell extracts, respectively.Applications for XRCC3 Antibody - BSA Free
Application
Recommended Usage
Western Blot
1:5000
Application Notes
This XRCC3 antibody is useful for Western blot, where a band can be seen at 38 kDa. No cross-reactivity was observed with XRCC2, RAD51, RAD51B, RAD51C, or RAD51D. The antibody is not applicable for Immunocytochemistry/Immunofluorescence.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using NB100-165 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: XRCC3
Alternate Names
CMM6, DNA repair protein XRCC3, X-ray repair complementing defective repair in Chinese hamster cells 3, X-ray repair cross-complementing protein 3
Gene Symbol
XRCC3
Additional XRCC3 Products
Product Documents for XRCC3 Antibody - BSA Free
Product Specific Notices for XRCC3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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