YAP1 Antibody (1A12) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22117

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Knockout Validated, Simple Western, Western Blot

Cited:

Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1A12

Format

BSA Free

Concentration

1.0 mg/ml

View all YAP1 Antibodies »

Product Specifications

Immunogen

This YAP1 antibody (1A12) was developed against recombinant human YAP1 protein expressed in E. coli. [UniProt# P46937]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for YAP1 Antibody (1A12) - BSA Free

Knockout Validated: YAP1 Antibody (1A12) - BSA Free [NBP2-22117]

Knockout Validated: YAP1 Antibody (1A12) - BSA Free [NBP2-22117]

Knockout Validated: YAP1 Antibody (1A12) [NBP2-22117] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:500 of Mouse Anti-Human YAP1 Monoclonal Antibody (Catalog # NBP2-22117) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific band was detected for YAP1 at approximately 75 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Immunocytochemistry/ Immunofluorescence: YAP1 Antibody (1A12) - BSA Free [NBP2-22117]

Immunocytochemistry/ Immunofluorescence: YAP1 Antibody (1A12) - BSA Free [NBP2-22117]

Immunocytochemistry/Immunofluorescence: YAP1 Antibody (1A12) - BSA Free [NBP2-22117] - Caco-2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with YAP1 Antibody [1A12] (NBP2-22117) at 1 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: YAP1 Antibody (1A12) - BSA Free [NBP2-22117]

Immunohistochemistry-Paraffin: YAP1 Antibody (1A12) - BSA Free [NBP2-22117]

Immunohistochemistry-Paraffin: YAP1 Antibody (1A12) [NBP2-22117] - Analysis of paraffin-embedded prostate cancer tissues using YAP1 mouse mAb with DAB staining. 1:200 - 1:1000
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Applications for YAP1 Antibody (1A12) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1-5 ug/mL

Immunohistochemistry-Paraffin

1:200-1:1000

Immunoprecipitation

reported in scientific literature (PMID 28230103)

Knockdown Validated

reported in scientific literature (PMID 28230103)

Simple Western

20 ug/mL

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: YAP1

The transcriptional coactivator Yes-associated protein (YAP), also known as Yes-associated protein 1 (YAP1), Yes-Associated Protein YAP65 Homolog, Protein Yorkie Homolog and YAP65 has a theoretical molecular weight of 65 kDa. The YAP1 gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. YAP1 shares homology with the WW domain of transcriptional co-activator with PDZ-binding motif (TAZ), which functions as a transcriptional co-activator by binding to the PPXY motif present in transcription factors and is likely involved in protein-protein interaction. YAP has been identified as a core regulatory mechanism that blocks mammalian glial cell proliferation and cellular reprogramming following damage (1).

YAP plays a role in the development and progression of multiple cancers as a transcriptional regulator of the Hippo signaling pathway. YAP1 encodes a nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis to play a pivotal role in controlling cell growth and organ size and has emerged as a key player in tumor suppression (2,3). Deregulation of the Hippo pathway causes tumor formation and malignancy, with YAP being a key oncogenic driver in liver carcinogenesis (2) and may function as a potential target for cancer treatment (3).

References

1. Rueda, E. M., Hall, B. M., Hill, M. C., Swinton, P. G., Tong, X., Martin, J. F., & Poche, R. A. (2019). The Hippo Pathway Blocks Mammalian Retinal Muller Glial Cell Reprogramming. Cell Rep, 27(6), 1637-1649.e1636. doi:10.1016/j.celrep.2019.04.047

2. Liu, A. M., Xu, M. Z., Chen, J., Poon, R. T., & Luk, J. M. (2010). Targeting YAP and Hippo signaling pathway in liver cancer. Expert Opin Ther Targets, 14(8), 855-868. doi:10.1517/14728222.2010.499361

3.Ye, S., & Eisinger-Mathason, T. S. (2016). Targeting the Hippo pathway: Clinical implications and therapeutics. Pharmacol Res, 103, 270-278. doi:10.1016/j.phrs.2015.11.025

Long Name

Yes-associated Protein 1

Alternate Names

YAP2, YAP65, YKI, Yorkie Homolog

Entrez Gene IDs

10413 (Human)

Gene Symbol

YAP1

UniProt

P46937

Additional YAP1 Products

Product Documents for YAP1 Antibody (1A12) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for YAP1 Antibody (1A12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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