YAP1 Antibody (2F12) - Isoform 5

Novus Biologicals, part of Bio-Techne | Catalog # H00010413-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Porcine, Bovine, Canine

Cited:

Human, Mouse, Bovine, Canine

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Western Blot

Cited:

Block/Neutralize, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, In vivo assay, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2F12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all YAP1 Antibodies »

Product Specifications

Immunogen

YAP1 (NP_006097, 53 a.a. ~ 161 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QIVHVRGDSETDLEALFNAVMNPKTANVPQTVPMRLRKLPDSFFKPPEPKSHSRQASTDAGTAGALTPQHVRAHSSPASLQLGAVSPGTLTPTGVVSGPAATPTAQHLR

Reactivity Notes

Mouse reactivity reported in the scientific literature (PMID: 23791527). Use in Canine reported in scientific literature (PMID:28793266). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions. Use in Bovine reported in secitific publication PMID: 32513530

Localization

Nucleoplasm, Nucleoli and cytoplasm

Specificity

Expected cross reactivity based on immunogen sequence isoforms: Isoform 1 (100%), Isoform 2 (100%), Isoform 3 (100%), Isoform 6 (100)%, Isoform 7 (100%), Isoform 8 (100%), Isoform 9 (100%)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for YAP1 Antibody (2F12) - Isoform 5

Knockdown Validated: YAP1 Antibody (2F12) - Isoform 5 [H00010413-M01]

Knockdown Validated: YAP1 Antibody (2F12) - Isoform 5 [H00010413-M01]

Knockdown Validated: YAP1 Antibody (2F12) - Isoform 5 [H00010413-M01] - YAP1 Antibody (2F12) [H00010413-M01] - JNK phosphorylation of YAP enhances the stabilization of delta-Np63alpha by direct binding. H357 cells were transfected with control siRNA (siCTL) or YAP siRNA (siYAP) and 72 h later the cells were harvested for IP of endogenous p63. p63 IP eluates and input lysates were analyzed by Western blotting using the indicated antibodies. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/cddis20107) licensed under a CC-BY license.
Western Blot: YAP1 Antibody (2F12)Isoform 5 [H00010413-M01]

Western Blot: YAP1 Antibody (2F12)Isoform 5 [H00010413-M01]

Western Blot: YAP1 Antibody (2F12) [H00010413-M01] - Analysis of YAP1 Antibody (2F12) Isoform 5 expression in Hela S3 N cells. Observed molecular weight 75 kDa.
Immunocytochemistry/ Immunofluorescence: YAP1 Antibody (2F12) - Isoform 5 [H00010413-M01]

Immunocytochemistry/ Immunofluorescence: YAP1 Antibody (2F12) - Isoform 5 [H00010413-M01]

Immunocytochemistry/Immunofluorescence: YAP1 Antibody (2F12) [H00010413-M01] - Analysis of monoclonal antibody to YAP1 on HeLa cell. Antibody concentration 30 ug/ml.
View 4 more images

Applications for YAP1 Antibody (2F12) - Isoform 5

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

30 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

3 ug/ml

Immunoprecipitation

1:10-1:500

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: YAP1

The transcriptional coactivator Yes-associated protein (YAP), also known as Yes-associated protein 1 (YAP1), Yes-Associated Protein YAP65 Homolog, Protein Yorkie Homolog and YAP65 has a theoretical molecular weight of 65 kDa. The YAP1 gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. YAP1 shares homology with the WW domain of transcriptional co-activator with PDZ-binding motif (TAZ), which functions as a transcriptional co-activator by binding to the PPXY motif present in transcription factors and is likely involved in protein-protein interaction. YAP has been identified as a core regulatory mechanism that blocks mammalian glial cell proliferation and cellular reprogramming following damage (1).

YAP plays a role in the development and progression of multiple cancers as a transcriptional regulator of the Hippo signaling pathway. YAP1 encodes a nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis to play a pivotal role in controlling cell growth and organ size and has emerged as a key player in tumor suppression (2,3). Deregulation of the Hippo pathway causes tumor formation and malignancy, with YAP being a key oncogenic driver in liver carcinogenesis (2) and may function as a potential target for cancer treatment (3).

References

1. Rueda, E. M., Hall, B. M., Hill, M. C., Swinton, P. G., Tong, X., Martin, J. F., & Poche, R. A. (2019). The Hippo Pathway Blocks Mammalian Retinal Muller Glial Cell Reprogramming. Cell Rep, 27(6), 1637-1649.e1636. doi:10.1016/j.celrep.2019.04.047

2. Liu, A. M., Xu, M. Z., Chen, J., Poon, R. T., & Luk, J. M. (2010). Targeting YAP and Hippo signaling pathway in liver cancer. Expert Opin Ther Targets, 14(8), 855-868. doi:10.1517/14728222.2010.499361

3.Ye, S., & Eisinger-Mathason, T. S. (2016). Targeting the Hippo pathway: Clinical implications and therapeutics. Pharmacol Res, 103, 270-278. doi:10.1016/j.phrs.2015.11.025

Long Name

Yes-associated Protein 1

Alternate Names

YAP2, YAP65, YKI, Yorkie Homolog

Entrez Gene IDs

10413 (Human); 363014 (Rat)

Gene Symbol

YAP1

OMIM

606608 (Human)

Additional YAP1 Products

Product Documents for YAP1 Antibody (2F12) - Isoform 5

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for YAP1 Antibody (2F12) - Isoform 5

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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