YAP1 Antibody (S06-5E9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-19805
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # S06-5E9
Concentration
0.3 mg/ml
Product Specifications
Immunogen
A synthetic peptide of human YAP1 (Uniprot # P46937)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for YAP1 Antibody (S06-5E9)
![Western Blot: YAP1 Antibody (S06-5E9) [NBP3-19805]](https://resources.bio-techne.com/images/products/YAP1-Antibody-S06-5E9-Western-Blot-NBP3-19805-img0001.jpg "Western Blot: YAP1 Antibody (S06-5E9) [NBP3-19805]")
Western Blot: YAP1 Antibody (S06-5E9) [NBP3-19805]
Western Blot: YAP1 Antibody (S06-5E9) [NBP3-19805] - Western blot detection of YAP1 in Hela cell lysates using NBP3-19805 (1:1000 diluted). Predicted band size: 55kDa. Observed band size: 70-75kDa.Applications for YAP1 Antibody (S06-5E9)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1/100
Western Blot
1/1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Concentration
0.3 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: YAP1
YAP plays a role in the development and progression of multiple cancers as a transcriptional regulator of the Hippo signaling pathway. YAP1 encodes a nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis to play a pivotal role in controlling cell growth and organ size and has emerged as a key player in tumor suppression (2,3). Deregulation of the Hippo pathway causes tumor formation and malignancy, with YAP being a key oncogenic driver in liver carcinogenesis (2) and may function as a potential target for cancer treatment (3).
References
1. Rueda, E. M., Hall, B. M., Hill, M. C., Swinton, P. G., Tong, X., Martin, J. F., & Poche, R. A. (2019). The Hippo Pathway Blocks Mammalian Retinal Muller Glial Cell Reprogramming. Cell Rep, 27(6), 1637-1649.e1636. doi:10.1016/j.celrep.2019.04.047
2. Liu, A. M., Xu, M. Z., Chen, J., Poon, R. T., & Luk, J. M. (2010). Targeting YAP and Hippo signaling pathway in liver cancer. Expert Opin Ther Targets, 14(8), 855-868. doi:10.1517/14728222.2010.499361
3.Ye, S., & Eisinger-Mathason, T. S. (2016). Targeting the Hippo pathway: Clinical implications and therapeutics. Pharmacol Res, 103, 270-278. doi:10.1016/j.phrs.2015.11.025
Long Name
Yes-associated Protein 1
Alternate Names
YAP2, YAP65, YKI, Yorkie Homolog
Gene Symbol
YAP1
Additional YAP1 Products
Product Documents for YAP1 Antibody (S06-5E9)
Product Specific Notices for YAP1 Antibody (S06-5E9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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