YAP1 [p Ser127] Antibody (SR2105)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21551

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-21551-100ul
NBP3-21551-50ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR2105

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all YAP1 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human phospho-YAP1 [Ser127] (Uniprot #: P46937)

Modification

p Ser 127

Specificity

Detects endogenous levels of phospho-YAP1 [Ser127]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for YAP1 [p Ser127] Antibody (SR2105)

Western Blot: YAP1 [p Ser 127] Antibody (SR2105) [NBP3-21551] -

Western Blot: YAP1 [p Ser 127] Antibody (SR2105) [NBP3-21551] - Western blot analysis using NBP3-21551 in (1) HeLa cell lysate; (2) HeLa cell lysate treated with FBS+calyculin A.

Immunohistochemistry: YAP1 [p Ser 127] Antibody (SR2105) [NBP3-21551] -

Immunohistochemistry: YAP1 [p Ser 127] Antibody (SR2105) [NBP3-21551] - Immunohistochemical analysis of paraffin-embedded human stomach, using NBP3-21551.

Applications for YAP1 [p Ser127] Antibody (SR2105)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:5000-1:20000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: YAP1

The transcriptional coactivator Yes-associated protein (YAP), also known as Yes-associated protein 1 (YAP1), Yes-Associated Protein YAP65 Homolog, Protein Yorkie Homolog and YAP65 has a theoretical molecular weight of 65 kDa. The YAP1 gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. YAP1 shares homology with the WW domain of transcriptional co-activator with PDZ-binding motif (TAZ), which functions as a transcriptional co-activator by binding to the PPXY motif present in transcription factors and is likely involved in protein-protein interaction. YAP has been identified as a core regulatory mechanism that blocks mammalian glial cell proliferation and cellular reprogramming following damage (1).

YAP plays a role in the development and progression of multiple cancers as a transcriptional regulator of the Hippo signaling pathway. YAP1 encodes a nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis to play a pivotal role in controlling cell growth and organ size and has emerged as a key player in tumor suppression (2,3). Deregulation of the Hippo pathway causes tumor formation and malignancy, with YAP being a key oncogenic driver in liver carcinogenesis (2) and may function as a potential target for cancer treatment (3).

References

1. Rueda, E. M., Hall, B. M., Hill, M. C., Swinton, P. G., Tong, X., Martin, J. F., & Poche, R. A. (2019). The Hippo Pathway Blocks Mammalian Retinal Muller Glial Cell Reprogramming. Cell Rep, 27(6), 1637-1649.e1636. doi:10.1016/j.celrep.2019.04.047

2. Liu, A. M., Xu, M. Z., Chen, J., Poon, R. T., & Luk, J. M. (2010). Targeting YAP and Hippo signaling pathway in liver cancer. Expert Opin Ther Targets, 14(8), 855-868. doi:10.1517/14728222.2010.499361

3.Ye, S., & Eisinger-Mathason, T. S. (2016). Targeting the Hippo pathway: Clinical implications and therapeutics. Pharmacol Res, 103, 270-278. doi:10.1016/j.phrs.2015.11.025

Long Name

Yes-associated Protein 1

Alternate Names

YAP2, YAP65, YKI, Yorkie Homolog

Gene Symbol

YAP1

Additional YAP1 Products

Product Documents for YAP1 [p Ser127] Antibody (SR2105)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for YAP1 [p Ser127] Antibody (SR2105)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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