Yes Antibody (2D5)
Novus Biologicals, part of Bio-Techne | Catalog # H00007525-M03
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2D5
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
YES1 (AAH48960, 1 a.a. ~ 543 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGCIKSKENKSPAIKYRPENTPEPVSTSVSHYGAEPTTVSPCPSSSAKGTAVNFSSLSMTPFGGSSGVTPFGGASSSFSVVPSSYPAGLTGGVTIFVALYDYEARTTEDLSFKKGERFQIINNTEGDWWEARSIATGKNGYIPSNYVAPADSIQAEEWYFGKMGRKDAERLLLNPGNQRGIFLVRESETTKGAYSLSIRDWDEIRGDNVKHYKIRKLDNGGYYITTRAQFDTLQKLVKHYTEHADGLCHKLTTVCPTVKPQTQGLAKDAWEIPRESLRLEVKLGQGCFGEVWMGTWNGTTKVAIKTLKPGTMMPEAFLQEAQIMKKLRHDKLVPLYAVVSEEPIYIVTEFMSKGSLLDFLKEGDGKYLKLPQLVDMAAQIADGMAYIERMNYIHRDLRAANILVGENLVCKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILQTELVTKGRVPYPGMVNREVLEQVERGYRMPCPQGCPESLHELMNLCWKKDPDERPTFEYIQSFLEDYFTATEPQYQPGENL
Specificity
YES1 - v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1 (2D5)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Yes Antibody (2D5)
![Western Blot: Yes Antibody (2D5) [H00007525-M03]](https://resources.bio-techne.com/images/products/Yes-Antibody-2D5-Western-Blot-H00007525-M03-img0005.jpg "Western Blot: Yes Antibody (2D5) [H00007525-M03]")
Western Blot: Yes Antibody (2D5) [H00007525-M03]
Western Blot: Yes Antibody (2D5) [H00007525-M03] - Analysis of YES1 expression in A-431 (Cat # L015V1).![Immunohistochemistry-Paraffin: Yes Antibody (2D5) [H00007525-M03]](https://resources.bio-techne.com/images/products/Yes-Antibody-2D5-Immunohistochemistry-Paraffin-H00007525-M03-img0003.jpg "Immunohistochemistry-Paraffin: Yes Antibody (2D5) [H00007525-M03]")
Immunohistochemistry-Paraffin: Yes Antibody (2D5) [H00007525-M03]
Immunohistochemistry-Paraffin: Yes Antibody (2D5) [H00007525-M03] - Analysis of monoclonal antibody to YES1 on formalin-fixed paraffin-embedded human smooth muscle. Antibody concentration 3 ug/ml
Sandwich ELISA: Yes Antibody (2D5) [H00007525-M03] - Detection limit for recombinant GST tagged YES1 is approximately 10ng/ml as a capture antibody.
Applications for Yes Antibody (2D5)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA and IHC-P.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Yes
Long Name
V-Yes-1 Yamaguchi Sarcoma Viral Oncogene Homolog 1
Alternate Names
YES1
Entrez Gene IDs
7525 (Human)
Gene Symbol
YES1
UniProt
Additional Yes Products
Product Documents for Yes Antibody (2D5)
Product Specific Notices for Yes Antibody (2D5)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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