Yes Antibody (2F4)
Novus Biologicals, part of Bio-Techne | Catalog # H00007525-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2F4
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
YES1 (AAH48960, 1 a.a. ~ 543 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGCIKSKENKSPAIKYRPENTPEPVSTSVSHYGAEPTTVSPCPSSSAKGTAVNFSSLSMTPFGGSSGVTPFGGASSSFSVVPSSYPAGLTGGVTIFVALYDYEARTTEDLSFKKGERFQIINNTEGDWWEARSIATGKNGYIPSNYVAPADSIQAEEWYFGKMGRKDAERLLLNPGNQRGIFLVRESETTKGAYSLSIRDWDEIRGDNVKHYKIRKLDNGGYYITTRAQFDTLQKLVKHYTEHADGLCHKLTTVCPTVKPQTQGLAKDAWEIPRESLRLEVKLGQGCFGEVWMGTWNGTTKVAIKTLKPGTMMPEAFLQEAQIMKKLRHDKLVPLYAVVSEEPIYIVTEFMSKGSLLDFLKEGDGKYLKLPQLVDMAAQIADGMAYIERMNYIHRDLRAANILVGENLVCKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILQTELVTKGRVPYPGMVNREVLEQVERGYRMPCPQGCPESLHELMNLCWKKDPDERPTFEYIQSFLEDYFTATEPQYQPGENL
Specificity
YES1 - v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Yes Antibody (2F4)
![Western Blot: Yes Antibody (2F4) [H00007525-M01]](https://resources.bio-techne.com/images/products/Yes-Antibody-2F4-Western-Blot-H00007525-M01-img0003.jpg "Western Blot: Yes Antibody (2F4) [H00007525-M01]")
Western Blot: Yes Antibody (2F4) [H00007525-M01]
Western Blot: Yes Antibody (2F4) [H00007525-M01] - Analysis of YES1 over-expressed 293 cell line, cotransfected with YES1 Validated Chimera RNAi ( Cat # H00007525-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with YES1 monoclonal antibody (M01) clone 2F4 (Cat # H00007525-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.![Western Blot: Yes Antibody (2F4) [H00007525-M01]](https://resources.bio-techne.com/images/products/Yes-Antibody-2F4-Western-Blot-H00007525-M01-img0004.jpg "Western Blot: Yes Antibody (2F4) [H00007525-M01]")
Western Blot: Yes Antibody (2F4) [H00007525-M01]
Western Blot: Yes Antibody (2F4) [H00007525-M01] - Analysis of YES1 expression in transfected 293T cell line by YES1 monoclonal antibody (M01), clone 2F4.Lane 1: YES1 transfected lysate(59.84 KDa).Lane 2: Non-transfected lysate.Applications for Yes Antibody (2F4)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Yes
Long Name
V-Yes-1 Yamaguchi Sarcoma Viral Oncogene Homolog 1
Alternate Names
YES1
Entrez Gene IDs
7525 (Human)
Gene Symbol
YES1
OMIM
164880 (Human)
UniProt
Additional Yes Products
Product Documents for Yes Antibody (2F4)
Product Specific Notices for Yes Antibody (2F4)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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