Yes Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85369

Novus Biologicals, part of Bio-Techne
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NBP1-85369
NBP1-85369-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

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Product Summary for Yes Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KSKENKSPAIKYRPENTPEPVSTSVSHYGAEPTTVSPCPSSSAKGTAVNFSSLS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Yes Antibody

Western Blot: Yes Antibody [NBP1-85369]

Western Blot: Yes Antibody [NBP1-85369]

Western Blot: Yes Antibody [NBP1-85369] - Analysis in human cell line U-2 OS.
Immunocytochemistry/ Immunofluorescence: Yes Antibody [NBP1-85369]

Immunocytochemistry/ Immunofluorescence: Yes Antibody [NBP1-85369]

Immunocytochemistry/Immunofluorescence: Yes Antibody [NBP1-85369] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
Immunohistochemistry-Paraffin: Yes Antibody [NBP1-85369]

Immunohistochemistry-Paraffin: Yes Antibody [NBP1-85369]

Immunohistochemistry-Paraffin: Yes Antibody [NBP1-85369] - Staining of human kidney shows moderate cytoplasmic positivity in tubule and glomerular cells.

Applications for Yes Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Yes

Yes1 is the cellular homolog of the Yamaguchi sarcoma virus oncogene. The encoded protein has tyrosine kinase activity and belongs to the src family of proteins. This gene lies in close proximity to thymidylate synthase gene on chromosome 18, and a corresponding pseudogene has been found on chromosome 22. [provided by RefSeq]

Long Name

V-Yes-1 Yamaguchi Sarcoma Viral Oncogene Homolog 1

Alternate Names

YES1

Gene Symbol

YES1

Additional Yes Products

Product Documents for Yes Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Yes Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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