YY1 Antibody (6J6L1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15779

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15779-100ul
NBP3-15779-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6J6L1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all YY1 Antibodies »

Product Summary for YY1 Antibody (6J6L1)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human YY1 (P25490). PGNKKWEQKQVQIKTLEGEFSVTMWSSDEKKDIDHETVVEEQIIGENSPPDYSEYMTGKKLPPGGIPGIDLSDPKQLAEFARMKPRKIKEDDAPRTIACPH

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for YY1 Antibody (6J6L1)

Western Blot: YY1 Antibody (6J6L1) [NBP3-15779]

Western Blot: YY1 Antibody (6J6L1) [NBP3-15779]

Western Blot: YY1 Antibody (6J6L1) [NBP3-15779] - Western blot analysis of extracts of various cell lines, using YY1 antibody (NBP3-15779) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: YY1 Antibody (6J6L1) [NBP3-15779]

Immunocytochemistry/ Immunofluorescence: YY1 Antibody (6J6L1) [NBP3-15779]

Immunocytochemistry/Immunofluorescence: YY1 Antibody (6J6L1) [NBP3-15779] - Immunofluorescence analysis of C6 cells using YY1 antibody (NBP3-15779). Blue: DAPI for nuclear staining.
Chromatin Immunoprecipitation: YY1 Antibody (6J6L1) [NBP3-15779] - Chromatin immunoprecipitation analysis of extracts of 293T cells, using YY1 antibody (NBP3-15779) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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Applications for YY1 Antibody (6J6L1)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:1000 - 1:5000

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunoprecipitation

1:1000 - 1:5000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: YY1

YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1.

Long Name

Yin-Yang-1

Alternate Names

DELTA, NF-E1, UCRBP

Gene Symbol

YY1

Additional YY1 Products

Product Documents for YY1 Antibody (6J6L1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for YY1 Antibody (6J6L1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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