What is the difference between the two ZAK antibodies: NBP1-90367 and NBP1-33137? According to the amino acid sequence, the molecular weight of ZAK-alpha is about 91KDa and ZAK-beta is about 51KDa. NBP1-90367 corresponds to ZAK aa 157-305, which is the common amino acid sequence, so it can detect ZAK-alpha and ZAK-beta simultaneously. In your datasheet, the predicted band size is 51KDa. Additionally, in your or my western blot, I can't detect band in 91KDa. There is only one band in 51KDa. Is it reasonable that the band locating in 51kDa is ZAK-beta?

Name: ZAK Antibody
Brand: Novus Biologicals
SKU: NBP1-33137

Both of these antibodies were raised to the N-terminal region shared by both isoforms 1&2. NBP1-33137 was generated using a partial recombinant protein containing a sequence within aa 18-254 of the human form. NBP1-90367 was developed to partial recombinant protein corresponding to aa 157-305. Based on the immunogen sequence, both antibodies are predicted to recognize both isoform 1 and isoform 2. There are two possible reasons why only one band shows on the WBs for each of these antibodies: 1. In tested tissues only one isoform is expressed. According to uniprot (http://www.uniprot.org/uniprot/Q9NYL2#expression) Isoform 2 is the predominant form in all tissues, except for liver, in which isoform 1 is more highly expressed. 2. These antibodies do indeed recognize only one isoform, despite shared immunogen sequence. We have not, however, tested these antibodies side by side on the same lysates, neither have we performed further specificity testing to rule out reactivity with the other isoform. Please also note that according to uniprot (http://www.uniprot.org/uniprot/Q9NYL2#sequences), there are actually 3 isoforms for MAP3K20, the third one is the shortest, yielding a protein of ~35kDa and NBP1-90367 is not going to recognize this isoform, but NBP1-33137 will.

ZAK Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33137

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ZAK Antibodies »

Product Summary for ZAK Antibody

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ZAK. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (85%).

Localization

Cytoplasm, Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

91 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ZAK Antibody

Western Blot: ZAK Antibody [NBP1-33137]

Western Blot: ZAK Antibody [NBP1-33137] - Sample (30 ug of whole cell lysate) A: Molt-4 7.5% SDS PAGE diluted at 1:1000

Immunocytochemistry/ Immunofluorescence: ZAK Antibody [NBP1-33137]

Immunocytochemistry/Immunofluorescence: ZAK Antibody [NBP1-33137] - Paraformaldehyde-fixed A431, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: ZAK Antibody [NBP1-33137]

Immunohistochemistry-Paraffin: ZAK Antibody [NBP1-33137] - Mahlarvu xenograft. ZAK antibody [N1N2], N-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Applications for ZAK Antibody

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ZAK

This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Alternate Names

AZK, EC 2.7.11, EC 2.7.11.25, HCCS-4, Human cervical cancer suppressor gene 4 protein, Leucine zipper- and sterile alpha motif-containing kinase, mitogen-activated protein kinase kinase kinase MLT, mixed lineage kinase 7, mixed lineage kinase with a leucine zipper and a sterile alpha motif, Mixed lineage kinase-related kinase, mixed lineage kinase-related kinase MRK-beta, MLK7, mlklak, MLK-like mitogen-activated protein triple kinase, MLK-related kinase, MLT, MLTK, MRKcervical cancer suppressor gene 4 protein, pk, protein kinase, sterile alpha motif and leucine zipper containing kinase AZK, Sterile alpha motif- and leucine zipper-containing kinase AZK

Gene Symbol

ZAK

Additional ZAK Products

Product Documents for ZAK Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for ZAK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.

ZAK Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33137

Key Product Details

Species Reactivity

Validated:

Predicted:

Applications

Label

Antibody Source

Concentration

View all ZAK Antibodies »

Product Summary for ZAK Antibody

Immunogen

Reactivity Notes

Localization

Clonality

Host

Isotype

Theoretical MW

Scientific Data Images for ZAK Antibody

Western Blot: ZAK Antibody [NBP1-33137]

Immunocytochemistry/ Immunofluorescence: ZAK Antibody [NBP1-33137]

Immunohistochemistry-Paraffin: ZAK Antibody [NBP1-33137]

Applications for ZAK Antibody

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Paraffin

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: ZAK

Alternate Names

Gene Symbol

Additional ZAK Products

Product Documents for ZAK Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for ZAK Antibody