ZAP70 [p Tyr352] Antibody (GT1686)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13623
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (100%), Chicken (100%), Xenopus (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # GT1686
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Tyr319 of human ZAP70. The exact sequence is proprietary.
Modification
p Tyr 352
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Centrifuge briefly prior to opening.
Scientific Data Images for ZAP70 [p Tyr352] Antibody (GT1686)
Western Blot: ZAP70 [p Tyr352] Antibody (GT1686) [NBP3-13623] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] (NBP3-13623) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: ZAP70 [p Tyr352] Antibody (GT1686) [NBP3-13623] - ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] detects ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) protein at cytoplasm by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) stained by ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] (NBP3-13623) diluted at 1:500. Blue: Fluoroshield with DAPI.
Western Blot: ZAP70 [p Tyr352] Antibody (GT1686) [NBP3-13623] - Untreated (-) and treated (+) Jurkat whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] (NBP3-13623) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Applications for ZAP70 [p Tyr352] Antibody (GT1686)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ZAP70
Long Name
Zeta-chain [TCR] Associated Protein Kinase 70 kDa
Alternate Names
mrtle, mur, SRK, STD, TZK
Gene Symbol
ZAP70
Additional ZAP70 Products
Product Documents for ZAP70 [p Tyr352] Antibody (GT1686)
Product Specific Notices for ZAP70 [p Tyr352] Antibody (GT1686)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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