ZEB1 Antibody (2A8A6) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23484

Novus Biologicals, part of Bio-Techne
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NBP2-23484
NBP2-23484SS
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

CyTOF-ready, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2A8A6

Format

BSA Free

Concentration

1.0 mg/ml

View all ZEB1 Antibodies »

Product Specifications

Immunogen

Partial recombinant human ZEB1 (between residues 800-1124) expressed in E. coli [Uniprot P37275]

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31331982).

Marker

Mesenchymal Cells Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for ZEB1 Antibody (2A8A6) - BSA Free

Western Blot: ZEB1 Antibody (2A8A6)BSA Free [NBP2-23484]

Western Blot: ZEB1 Antibody (2A8A6)BSA Free [NBP2-23484]

Western Blot: ZEB1 Antibody (2A8A6) [NBP2-23484] - CAFs induced miR-33b downregulation and promoted the EMT phenotype in lung cancer cells. The protein expression levels of E-cadherin, Vimentin, ZEB1, Twist1 and Snail1 in the lung cancer cells co-cultured with CAFs were detected by immunoblotting, using GAPDH protein as the loading control. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.23082), licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (2A8A6) - BSA Free [NBP2-23484]

Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (2A8A6) - BSA Free [NBP2-23484]

Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (2A8A6) [NBP2-23484] - ZEB1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunohistochemistry: ZEB1 Antibody (2A8A6) - BSA Free [NBP2-23484]

Immunohistochemistry: ZEB1 Antibody (2A8A6) - BSA Free [NBP2-23484]

Immunohistochemistry: ZEB1 Antibody (2A8A6) [NBP2-23484] - IHC analysis of paraffin-embedded human cervical cancer tissues with DAB staining.
View 6 more images

Applications for ZEB1 Antibody (2A8A6) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1: 500 - 1:2000. Use reported by customer review and in scientific literature (PMID 29383119)
Application Notes
This antibody has had weak results in Western blot.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-23484 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZEB1

ZEB1 (zinc-finger E-box-binding homeobox 1), also known as TCF8 (transcription factor 8), is a delta-EF1/ZFH-1 C2H2-type zinc finger that bears seven C2H2-type zinc fingers and one homeobox DNA-binding domain. ZEB1 is a transcriptional repressor of interleukin-2 (IL-2). Mutations in the ZEB1 gene are associated with posterior corneal dystrophy type 3 (PPC3) a rare disorder characterized by metaplasia and overgrowth of corneal endothelial cells. ZEB1 appears to be critical to epithelial to mesenchymal transitions (EMT) during development and cancer metastasis. As a transcription factor involved in EMT, ZEB1 promotes EMT by repressing genes associated with the epithelial phenotype and activates genes associated with the mesenchymal phenotype.

Long Name

Zinc Finger E-box Binding Homeobox 1

Alternate Names

AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP

Entrez Gene IDs

6935 (Human)

Gene Symbol

ZEB1

UniProt

P37275

Additional ZEB1 Products

Product Documents for ZEB1 Antibody (2A8A6) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ZEB1 Antibody (2A8A6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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