Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3G6

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all ZEB1 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-350 of human ZEB1 (NP_110378) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This ZEB1 antibody does not cross-react with ZEB2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

123.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ZEB1 Antibody (OTI3G6) - Azide and BSA Free

Western Blot: ZEB1 Antibody (OTI3G6)Azide and BSA Free [NBP2-74910]

Western Blot: ZEB1 Antibody (OTI3G6)Azide and BSA Free [NBP2-74910]

Western Blot: ZEB1 Antibody (OTI3G6) - Azide and BSA Free [NBP2-74910] - Cross-reactivity test with ZEB2: Analysis of HEK293T cells were transfected with plasmids overexpressing either ZEB2 (left) or ZEB1 (right).
Immunohistochemistry: ZEB1 Antibody (OTI3G6) - Azide and BSA Free [NBP2-74910]

Immunohistochemistry: ZEB1 Antibody (OTI3G6) - Azide and BSA Free [NBP2-74910]

Immunohistochemistry: ZEB1 Antibody (OTI3G6) - Azide and BSA Free [NBP2-74910] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: ZEB1 Antibody (OTI3G6)Azide and BSA Free [NBP2-74910]

Western Blot: ZEB1 Antibody (OTI3G6)Azide and BSA Free [NBP2-74910]

Western Blot: ZEB1 Antibody (OTI3G6) - Azide and BSA Free [NBP2-74910] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
View 5 more images

Applications for ZEB1 Antibody (OTI3G6) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ZEB1

ZEB1 encodes a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene (MIM 147680) expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site (Williams et al., 1991 [PubMed 1840704]).[supplied by OMIM]

Long Name

Zinc Finger E-box Binding Homeobox 1

Alternate Names

AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP

Gene Symbol

ZEB1

Additional ZEB1 Products

Product Documents for ZEB1 Antibody (OTI3G6) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ZEB1 Antibody (OTI3G6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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