ZEB2 Antibody (CL13467) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43869

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL13467

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all ZEB2/SIP1 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of O60315, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for ZEB2 Antibody (CL13467) - Azide and BSA Free

ZEB2 Antibody (CL13467) - Azide and BSA Free Immunohistochemistry-Paraffin: ZEB2 Antibody [NBP3-43869]

Immunohistochemistry-Paraffin: ZEB2 Antibody [NBP3-43869]

Staining of human anaplastic oligodendroglioma shows strong nuclear positivity in tumor cells.
ZEB2 Antibody (CL13467) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: ZEB2 Antibody [NBP3-43869]

Immunocytochemistry/ Immunofluorescence: ZEB2 Antibody [NBP3-43869]

Staining in U-87 cell line with Anti-ZEB2 monoclonal antibody) .
ZEB2 Antibody (CL13467) - Azide and BSA Free Immunohistochemistry-Paraffin: ZEB2 Antibody [NBP3-43869]

Immunohistochemistry-Paraffin: ZEB2 Antibody [NBP3-43869]

Analysis in human cerebral cortex and skeletal muscle tissues using NBP3-43869 antibody. Corresponding ZEB2 RNA-seq data are presented for the same tissues.
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Applications for ZEB2 Antibody (CL13467) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments, For Immunocytochemistry/ Immunofluorescence/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZEB2/SIP1

The ZEB2 gene (also known as SIP1 and SMADIP1) is a member of the delta-EF1 (ZEB1; MIM 189909)/Zfh1 family of 2-handed zinc finger/homeodomain proteins. SMADIP1 interacts with receptor-mediated, activated full-length SMADs (see MIM 605568) (Verschueren et al., 1999 [PubMed 10400677]).[supplied by OMIM]

Alternate Names

HSPC082, SIP1, SMADIP1, ZFHX1B

Gene Symbol

ZEB2

Additional ZEB2/SIP1 Products

Product Documents for ZEB2 Antibody (CL13467) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ZEB2 Antibody (CL13467) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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