ZFAND5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80609

Novus Biologicals, part of Bio-Techne
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NBP1-80609
NBP1-80609-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Predicted:

Mouse (97%), Rat (96%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ZFAND5 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TASGSNSPTSDSASVQRADTSLNNCEGAAGSTSEKSRNVPVAALPVTQQMTEMSISREDKITTPKTEVSEPVVTQPSPSVSQPSTSQSEEKAPELPKPKK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZFAND5 Antibody

Western Blot: ZFAND5 Antibody [NBP1-80609]

Western Blot: ZFAND5 Antibody [NBP1-80609]

Western Blot: ZFAND5 Antibody [NBP1-80609] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: ZFAND5 Antibody [NBP1-80609]

Immunocytochemistry/ Immunofluorescence: ZFAND5 Antibody [NBP1-80609]

Immunocytochemistry/Immunofluorescence: ZFAND5 Antibody [NBP1-80609] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: ZFAND5 Antibody [NBP1-80609]

Immunohistochemistry-Paraffin: ZFAND5 Antibody [NBP1-80609]

Immunohistochemistry-Paraffin: ZFAND5 Antibody [NBP1-80609] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.
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Applications for ZFAND5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZFAND5

ZFAND5 inhibits NF-kappa-B activation triggered by overexpression of RIPK1 and TRAF6 but not of RELA. Inhibits also tumor necrosis (TNF), IL-1 and TLR4-induced NF-kappa-B activation in a dose-dependent manner. Overexpression sensitizes cells to TNF-induced apopt

Alternate Names

Zinc finger A20 domain-containing protein 2, Zinc finger protein 216ZA20D2, zinc finger, AN1-type domain 5, ZNF216A20 domain containing 2

Entrez Gene IDs

7763 (Human)

Gene Symbol

ZFAND5

Additional ZFAND5 Products

Product Documents for ZFAND5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ZFAND5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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