Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Zfp219 Antibodies »

Product Specifications

Immunogen

Antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human ZFP219. The immunogen is located within amino acids 90 - 140 of ZFP219. Amino Acid Squence: SHLRTHQPERPRSPAARL

Specificity

At least two isoforms of ZFP219 are known to exist; this antibody will recognize both.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Zfp219 Antibody - BSA Free

Western Blot: Zfp219 AntibodyBSA Free [NBP1-76550]

Western Blot: Zfp219 AntibodyBSA Free [NBP1-76550]

Western Blot: Zfp219 Antibody [NBP1-76550] - Analysis in EL4 cell lysate with antibody at 1 ug/mL.
Zfp219 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Zfp219 Antibody - BSA Free [NBP1-76550] -

Immunocytochemistry/ Immunofluorescence: Zfp219 Antibody - BSA Free [NBP1-76550] - Immunofluorescence of Zfp219 in mouse brain tissue with Zfp219 antibody at 20 ug/mL.
Zfp219 Antibody - BSA Free

Immunohistochemistry: Zfp219 Antibody - BSA Free [NBP1-76550] -

Immunohistochemistry: Zfp219 Antibody - BSA Free [NBP1-76550] - Immunohistochemistry of Zfp219 in mouse brain tissue with Zfp219 antibody at 5 ug/mL.

Applications for Zfp219 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Zfp219

ZFP219 is a developmentally regulated member of the Kruppel-like zinc finger gene family that is thought to function as a transcriptional repressor. Yeast two-hybrid screening showed association with Sox9, a transcription factor that is essential for chondrogenesis. ZFP219 is specifically expressed in the developing limb buds and colocalizes with Sox9 in the nucleus. Knockdown of ZFP219 expression decreased Sox9-induced mRNA expression, and a dominant-negative mutant of ZFP219 inhibited Bmp2-induced chondrocyte differentiation, suggesting that ZFP219 plays an important role as a transcriptional partner of Sox9 in the regulation of chondrocyte differentiation.

Alternate Names

FLJ14457, MGC126229, MGC126230, zinc finger protein 514

Gene Symbol

ZNF219

UniProt

NP_001095142 (Human)

Additional Zfp219 Products

Product Documents for Zfp219 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Zfp219 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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