ZHX3 Antibody (PCRP-ZHX3-1G3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13889

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # PCRP-ZHX3-1G3

Concentration

0.2 mg/ml

View all ZHX3 Antibodies »

Product Summary for ZHX3 Antibody (PCRP-ZHX3-1G3)

Immunogen

Recombinant full-length human ZHX3 protein (Uniprot: Q9H4I2)

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

105 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14230)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for ZHX3 Antibody (PCRP-ZHX3-1G3)

Immunocytochemistry/ Immunofluorescence: ZHX3 Antibody (PCRP-ZHX3-1G3) [NBP3-13889]

Immunocytochemistry/ Immunofluorescence: ZHX3 Antibody (PCRP-ZHX3-1G3) [NBP3-13889]

Immunocytochemistry/Immunofluorescence: ZHX3 Antibody (PCRP-ZHX3-1G3) [NBP3-13889] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using ZHX3 Antibody (PCRP-ZHX3-1G3) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Immunohistochemistry-Paraffin: ZHX3 Antibody (PCRP-ZHX3-1G3) [NBP3-13889]

Immunohistochemistry-Paraffin: ZHX3 Antibody (PCRP-ZHX3-1G3) [NBP3-13889]

Immunohistochemistry-Paraffin: ZHX3 Antibody (PCRP-ZHX3-1G3) [NBP3-13889] - Formalin-fixed, paraffin-embedded human thyroid stained with ZHX3 antibody (PCRP-ZHX3-1G3)at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Flow Cytometry: ZHX3 Antibody (PCRP-ZHX3-1G3) [NBP3-13889]

Flow Cytometry: ZHX3 Antibody (PCRP-ZHX3-1G3) [NBP3-13889]

Flow Cytometry: ZHX3 Antibody (PCRP-ZHX3-1G3) [NBP3-13889] - Flow cytometric analysis of PFA-fixed HeLa cells. ZHX3 antibody (PCRP-ZHX3-1G3) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
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Applications for ZHX3 Antibody (PCRP-ZHX3-1G3)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Immunoprecipitation

1-2 ug/100-500 ug protein

Western Blot

1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA

Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: ZHX3

ZHX3 encodes a member of the zinc fingers and homeoboxes (ZHX) gene family. The encoded protein contains two C2H2-type zinc fingers and five homeodomains and forms a dimer with itself or with zinc fingers and homeoboxes family member 1. In the nucleus, the dimerized protein interacts with the A subunit of the ubiquitous transcription factor nuclear factor-Y and may function as a transcriptional repressor.

Alternate Names

FLJ93221, Sp38, zona pellucida binding protein, zona pellucida-binding protein 1, ZPBP1zona pellucida binding protein 1

Gene Symbol

ZHX3

UniProt

Q9H4I2 (Human)

Additional ZHX3 Products

Product Documents for ZHX3 Antibody (PCRP-ZHX3-1G3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ZHX3 Antibody (PCRP-ZHX3-1G3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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