ZHX3 Antibody (PCRP-ZHX3-1G3) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14230
![Protein Array: ZHX3 Antibody (PCRP-ZHX3-1G3)Azide and BSA Free [NBP3-14230]](https://resources.bio-techne.com/images/products/ZHX3-Antibody-PCRP-ZHX3-1G3-Azide-and-BSA-Free-Protein-Array-NBP3-14230-img0001.jpg "Protein Array: ZHX3 Antibody (PCRP-ZHX3-1G3)Azide and BSA Free [NBP3-14230]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Protein Array, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # PCRP-ZHX3-1G3
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for ZHX3 Antibody (PCRP-ZHX3-1G3) - Azide and BSA Free
Immunogen
Recombinant full-length human ZHX3 protein (Uniprot: Q9H4I2)
Localization
Nucleus
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
105 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13889).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for ZHX3 Antibody (PCRP-ZHX3-1G3) - Azide and BSA Free
Protein Array: ZHX3 Antibody (PCRP-ZHX3-1G3)Azide and BSA Free [NBP3-14230]
Protein Array: ZHX3 Antibody (PCRP-ZHX3-1G3) - Azide and BSA Free [NBP3-14230] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ZHX3 antibody (PCRP-ZHX3-1G3).
ZHX3 Antibody (PCRP-ZHX3-1G3)
Formalin-fixed, paraffin-embedded human thyroid stained with ZHX3 antibody (PCRP-ZHX3-1G3)at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for ZHX3 Antibody (PCRP-ZHX3-1G3) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Immunoprecipitation
1-2 ug per 100-500 ug protein
Western Blot
1-2 ug/ml
Application Notes
ELISA: For coating, order this antibody without BSA
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: ZHX3
Alternate Names
FLJ93221, Sp38, zona pellucida binding protein, zona pellucida-binding protein 1, ZPBP1zona pellucida binding protein 1
Gene Symbol
ZHX3
Additional ZHX3 Products
Product Documents for ZHX3 Antibody (PCRP-ZHX3-1G3) - Azide and BSA Free
Product Specific Notices for ZHX3 Antibody (PCRP-ZHX3-1G3) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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