ZIC1 Antibody (6B5O9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15774

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15774-100ul
NBP3-15774-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6B5O9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ZIC1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ZIC1 (Q15915). MLLDAGPQYPAIGVTTFGASRHHSAGDVAERDVGLGINPFADGMGAFKLNPSSHELASAGQTAFTSQAPGYAAAAALGHHHHPGHVGSYSSAAFNSTRDF

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZIC1 Antibody (6B5O9)

Western Blot: ZIC1 Antibody (6B5O9) [NBP3-15774]

Western Blot: ZIC1 Antibody (6B5O9) [NBP3-15774]

Western Blot: ZIC1 Antibody (6B5O9) [NBP3-15774] - Western blot analysis of extracts of various cell lines, using ZIC1 Rabbit mAb (NBP3-15774) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Applications for ZIC1 Antibody (6B5O9)

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ZIC1

The Zic-1 gene, which encodes a zinc finger protein, is expressed in the developing or matured central nervous system in a highly restricted manner. The Zic gene is expressed in granule cells that make synaptic contact with Purkinje cells. Clearly Zic-1 is a gene critical to cerebellar pattern formation. The expression of Zic genes is first detected at gastrulation and at neurulation, becomes restricted to the dorsal neural ectoderm and the dorsal paraxial mesoderm. Zic-2 and Zic-3 are highly similar genes, especially in their product's zinc finger motif and by comparison of their genomic organization in that they share common exon-intron boundaries and belong to the same gene family. By comparison in function, Zic-2 is essential for the formation of the brain and Zic-3 is important for right and left axis formation. The Zic-1 gene has been mapped to chromosome 9 in mouse. The 5' flanking region of the Zic-1 gene contains a region-specific enhancer determined to be essential in in vivo and in vitro deletion analysis. The temporal profile of mRNA expression differs for each of the Zic gene products. The Drosophila odd-paired gene is highly homologous to the Zic gene family.

Long Name

Zic Family Member 1

Alternate Names

ZNF201

Gene Symbol

ZIC1

Additional ZIC1 Products

Product Documents for ZIC1 Antibody (6B5O9)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ZIC1 Antibody (6B5O9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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