ZKSCAN3 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31566

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all ZKSCAN3 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZKSCAN3. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ZKSCAN3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ZKSCAN3 Antibody - BSA Free [NBP1-31566]

Immunocytochemistry/ Immunofluorescence: ZKSCAN3 Antibody - BSA Free [NBP1-31566]

Immunocytochemistry/Immunofluorescence: ZKSCAN3 Antibody [NBP1-31566] - PLX4720 induces JNK2/p38 MAPK-dependent cytoplasmic translocation of ZKSCAN3. Representative confocal images of subcellular translocation of endogenous TFEB (green) and ZKSCAN3 (red) in A375 cells treated with PLX4720 (1 i1/4M, 12 h). n = 3 independent experiments. Image collected and cropped by Citeab from the following publication (Transcriptional regulation of autophagy-lysosomal function in BRAF-driven melanoma progression and chemoresistance. Nat Commun (2019) licensed under a CC-BY license.
Western Blot: ZKSCAN3 AntibodyBSA Free [NBP1-31566]

Western Blot: ZKSCAN3 AntibodyBSA Free [NBP1-31566]

Western Blot: ZKSCAN3 Antibody [NBP1-31566] - Western Blot: ZKSCAN3 Antibody [NBP1-31566] - Immunoblots for ZKSCAN3 in cytoplasmic and nuclear fractions of A375 cells treated with DMSO, PLX4720 (1 uM, 12 h), or Torin1 (1 uM, 3 h). Lamin B1 serves as the control for the nuclear fractions, whereas LAMP1 and Tubulin are the control of the cytoplasmic fractions. Note that PLX4720 induces cytoplasmic enrichment of ZKSCAN3, whereas Torin1 has no effect on its nucleocytoplasmic redistribution. Image collected and cropped by Citeab from the following publication (Transcriptional regulation of autophagy-lysosomal function in BRAF-driven melanoma progression and chemoresistance. Nat Commun (2019) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: ZKSCAN3 Antibody - BSA Free [NBP1-31566]

Immunohistochemistry-Paraffin: ZKSCAN3 Antibody - BSA Free [NBP1-31566]

Immunohistochemistry-Paraffin: ZKSCAN3 Antibody [NBP1-31566] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of human lung using ZKSCAN3 antibody at 1:200 dilution. The primary antibody binding to ZKSCAN3 was detected using HRP conjugated anti-rabbit secondary antibody with DAB reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. Strong nuclear reactivity was observed.
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Applications for ZKSCAN3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ZKSCAN3

ZKSCAN3 may be involved in transcriptional regulation

Alternate Names

dJ874C20.1, FLJ33906, Zf47, ZFP47, zfp-47, Zinc finger and SCAN domain-containing protein 13, Zinc finger protein 306KIAA0426, Zinc finger protein 309ZNF306ZFP306, Zinc finger protein 47 homolog, zinc finger protein with KRAB and SCAN domains 3, zinc finger protein zfp47, zinc finger with KRAB and SCAN domains 3, ZNF309, ZSCAN13

Entrez Gene IDs

80317 (Human)

Gene Symbol

ZKSCAN3

UniProt

Q9BRR0 (Human)

Additional ZKSCAN3 Products

Product Documents for ZKSCAN3 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ZKSCAN3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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