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ZNF143 Antibody (2B4)

Novus Biologicals, part of Bio-Techne | Catalog # H00007702-M01

Novus Biologicals, part of Bio-Techne
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H00007702-M01

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Chemotaxis, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2B4

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Product Specifications

Immunogen

ZNF143 (AAH20219, 1 a.a. ~ 626 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTEFPGGGMEAQHVTLCLTEAVTVADGDNLENMEGVSLQAVTLADGSTAYIQHNSKDAKLIDGQVIQLEDGSAAYVQHVPIPKSTGDSLRLEDGQAVQLEDGTTAFIHHTSKDSYDQSALQAVQLEDGTTAYIHHAVQVPQSDTILAIQADGTVAGLHTGDATIDPDTISALEQYAAKVSIDGSESVAGTGMIGENEQEKKMQIVLQGHATRVTAKSQQSGEKAFRCEYDGCGKLYTTAHHLKVHERSHTGDRPYQCEHAGCGKAFATGYGLKSHVRTHTGEKPYRCSEDNCTKSFKTSGDLQKHIRTHTGERPFKCPFEGCGRSFTTSNIRKVHVRTHTGERPYYCTEPGCGRAFASATNYKNHVRIHTGEKPYVCTVPGCDKRFTEYSSLYKHHVVHTHSKPYNCNHCGKTYKQISTLVMHKRTAHNDTEPIEEEQEAFFEPPPGQGEDVLKGSQITYVTGVEGDDVVSTQVATVTQSGLSQQVTLISQDGTQHVNISQADMQAIGNTITMVTQDGTPITVPAHDAVISSAGTHSVAMVTAEGTEGQQVAIVAQDLAAFHTASSEMGHQQHSHHLVTTETRPLTLVATSNGTQIAVQLGEQPSLEEAIRIASRIQQGETPGLDD

Specificity

ZNF143 - zinc finger protein 143 (clone pHZ-1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for ZNF143 Antibody (2B4)

Western Blot: ZNF143 Antibody (2B4) [H00007702-M01]

Western Blot: ZNF143 Antibody (2B4) [H00007702-M01]

Western Blot: ZNF143 Antibody (2B4) [H00007702-M01] - ZNF143 monoclonal antibody (M01), clone 2B4 Analysis of ZNF143 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: ZNF143 Antibody (2B4) [H00007702-M01]

Immunocytochemistry/ Immunofluorescence: ZNF143 Antibody (2B4) [H00007702-M01]

Immunocytochemistry/Immunofluorescence: ZNF143 Antibody (2B4) [H00007702-M01] - Analysis of monoclonal antibody to ZNF143 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: ZNF143 Antibody (2B4) [H00007702-M01]

Immunohistochemistry-Paraffin: ZNF143 Antibody (2B4) [H00007702-M01]

Immunohistochemistry-Paraffin: ZNF143 Antibody (2B4) [H00007702-M01] - Analysis of monoclonal antibody to ZNF143 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma tissue. Antibody concentration 3 ug/ml.

Applications for ZNF143 Antibody (2B4)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody reactivity against cell lysate and recombinant protein for WB. IT has been used for IF, IHC-P, and ELISA. ChIP usage reported in scientific literature (PMID: 25645053).

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ZNF143

ZNF143 is a human homolog of Xenopus transcriptional activator staf that is involved in selenocystyl tRNA transcription which binds to the SPH motif of small nuclear RNA (snRNA) gene promoters.

Long Name

Zinc Finger Protein 143

Alternate Names

pHZ-1, SBF, STAF

Entrez Gene IDs

7702 (Human)

Gene Symbol

ZNF143

OMIM

603433 (Human)

Additional ZNF143 Products

Product Documents for ZNF143 Antibody (2B4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ZNF143 Antibody (2B4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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