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ZNF179 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13236

Novus Biologicals, part of Bio-Techne
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NBP2-13236
NBP2-13236-25ul

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (93%), Rat (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: LAQEIKNLSGWMGRTGPGFTSPDEMAAQLHDLRKVEAAKREFEEYVRQQDVATKRIFSALRVLPDTMRNLLSTQKDAILARHGVALLCKGRDQTLEALEAELQATAKAFMDSYT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZNF179 Antibody

Western Blot: ZNF179 Antibody [NBP2-13236]

Western Blot: ZNF179 Antibody [NBP2-13236]

Western Blot: ZNF179 Antibody [NBP2-13236] - Analysis in control (vector only transfected HEK293T lysate) and RNF112 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: ZNF179 Antibody [NBP2-13236]

Immunocytochemistry/ Immunofluorescence: ZNF179 Antibody [NBP2-13236]

Immunocytochemistry/Immunofluorescence: ZNF179 Antibody [NBP2-13236] - Immunofluorescent staining of human cell line SH-SY5Y shows localization to nuclear speckles.
Immunohistochemistry-Paraffin: ZNF179 Antibody [NBP2-13236]

Immunohistochemistry-Paraffin: ZNF179 Antibody [NBP2-13236]

Immunohistochemistry-Paraffin: ZNF179 Antibody [NBP2-13236] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.

Applications for ZNF179 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZNF179

ZNF179 encodes a member of the RING finger protein family of transcription factors. The protein is primarily expressed in brain. The gene is located within the Smith-Magenis syndrome region on chromosome 17.

Alternate Names

BFPZinc finger protein 179ZNF179Brain finger protein, FLJ99413, ring finger protein 112

Gene Symbol

RNF112

Additional ZNF179 Products

Product Documents for ZNF179 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ZNF179 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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