Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3G5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all ZNF217 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 730-1048 of human ZNF217(NP_006517) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

115.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ZNF217 Antibody (OTI3G5) - Azide and BSA Free

Western Blot: ZNF217 Antibody (OTI3G5)Azide and BSA Free [NBP2-74933]

Western Blot: ZNF217 Antibody (OTI3G5)Azide and BSA Free [NBP2-74933]

Western Blot: ZNF217 Antibody (OTI3G5) - Azide and BSA Free [NBP2-74933] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY ZNF217 ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti ZNF217.(1:200
Immunohistochemistry: ZNF217 Antibody (OTI3G5) - Azide and BSA Free [NBP2-74933]

Immunohistochemistry: ZNF217 Antibody (OTI3G5) - Azide and BSA Free [NBP2-74933]

Immunohistochemistry: ZNF217 Antibody (OTI3G5) - Azide and BSA Free [NBP2-74933] - Staining of paraffin-embedded Human lung tissue within the normal limits using anti ZNF217 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,1:150)
Immunohistochemistry: ZNF217 Antibody (OTI3G5) - Azide and BSA Free [NBP2-74933]

Immunohistochemistry: ZNF217 Antibody (OTI3G5) - Azide and BSA Free [NBP2-74933]

Immunohistochemistry: ZNF217 Antibody (OTI3G5) - Azide and BSA Free [NBP2-74933] - Staining of paraffin-embedded Human Kidney tissue within the normal limits using anti ZNF217 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150)
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Applications for ZNF217 Antibody (OTI3G5) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ZNF217

ZNF217 binds to the promoters of target genes and functions as repressor. Promotes cell proliferation and antagonizes cell death. Promotes phosphorylation of AKT1 at 'Ser-473'

Alternate Names

DKFZp781O2147, KIAA0335, MGC125707, MGC125710, zinc finger protein 518, zinc finger protein 518A, ZNF518

Gene Symbol

ZNF217

Additional ZNF217 Products

Product Documents for ZNF217 Antibody (OTI3G5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ZNF217 Antibody (OTI3G5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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