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ZNF259 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38172

Novus Biologicals, part of Bio-Techne
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NBP2-38172
NBP2-38172-25ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for ZNF259 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: RYTLSVRALEDMNREVVKTDSAATRIPELDFEIPAFSQKGALTTVEGLITRAISGLEQDQPARRANKDATAERIDEFIVKLKELKQV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZNF259 Antibody

Immunocytochemistry/ Immunofluorescence: ZNF259 Antibody [NBP2-38172]

Immunocytochemistry/ Immunofluorescence: ZNF259 Antibody [NBP2-38172]

Immunocytochemistry/Immunofluorescence: ZNF259 Antibody [NBP2-38172] - Staining of human cell line CACO-2 shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ZNF259 Antibody [NBP2-38172]

Immunohistochemistry-Paraffin: ZNF259 Antibody [NBP2-38172]

Immunohistochemistry-Paraffin: ZNF259 Antibody [NBP2-38172] - Staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
Simple Western: ZNF259 Antibody [NBP2-38172]

Simple Western: ZNF259 Antibody [NBP2-38172]

Simple Western: ZNF259 Antibody [NBP2-38172] - Simple Western lane view shows a specific band for ZNF259 in 0.2 mg/ml of HT-29 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation systems.

Applications for ZNF259 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Simple Western

1:25
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZNF259

ZNF259 may be a signaling molecule that communicates mitogenic signals from the cytoplasm to the nucleus

Alternate Names

ERP1, EZFIT-related protein 1, KIAA1396MGC150657, MGC150658, Z1971, zinc finger protein 471

Gene Symbol

ZPR1

UniProt

Additional ZNF259 Products

Product Documents for ZNF259 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ZNF259 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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