ZNF259 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38172
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: RYTLSVRALEDMNREVVKTDSAATRIPELDFEIPAFSQKGALTTVEGLITRAISGLEQDQPARRANKDATAERIDEFIVKLKELKQV
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (83%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ZNF259 Antibody
Immunocytochemistry/ Immunofluorescence: ZNF259 Antibody [NBP2-38172]
Immunocytochemistry/Immunofluorescence: ZNF259 Antibody [NBP2-38172] - Staining of human cell line CACO-2 shows localization to vesicles. Antibody staining is shown in green.Immunohistochemistry-Paraffin: ZNF259 Antibody [NBP2-38172]
Immunohistochemistry-Paraffin: ZNF259 Antibody [NBP2-38172] - Staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.Simple Western: ZNF259 Antibody [NBP2-38172]
Simple Western: ZNF259 Antibody [NBP2-38172] - Simple Western lane view shows a specific band for ZNF259 in 0.2 mg/ml of HT-29 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation systems.Applications for ZNF259 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Simple Western
1:25
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ZNF259
Alternate Names
ERP1, EZFIT-related protein 1, KIAA1396MGC150657, MGC150658, Z1971, zinc finger protein 471
Gene Symbol
ZPR1
UniProt
Additional ZNF259 Products
Product Documents for ZNF259 Antibody
Product Specific Notices for ZNF259 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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