ZNF704 Antibody (PCRP-ZNF704-3C10)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13765
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2C Clone # PCRP-ZNF704-3C10
Concentration
0.2 mg/ml
Product Summary for ZNF704 Antibody (PCRP-ZNF704-3C10)
Immunogen
Recombinant full-length human ZNF704 protein (Uniprot: Q6ZNC4)
Localization
Nucleus
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2C
Theoretical MW
45.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14168)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for ZNF704 Antibody (PCRP-ZNF704-3C10)
Immunocytochemistry/ Immunofluorescence: ZNF704 Antibody (PCRP-ZNF704-3C10) [NBP3-13765]
Immunocytochemistry/Immunofluorescence: ZNF704 Antibody (PCRP-ZNF704-3C10) [NBP3-13765] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using ZNF704 Antibody (PCRP-ZNF704-3C10) followed by goat anti-mouse IgG-CF488 (green). CF640 phalloidin (red).Immunohistochemistry-Paraffin: ZNF704 Antibody (PCRP-ZNF704-3C10) [NBP3-13765]
Immunohistochemistry-Paraffin: ZNF704 Antibody (PCRP-ZNF704-3C10) [NBP3-13765] - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with ZNF704 antibody (PCRP-ZNF704-3C10). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.Flow Cytometry: ZNF704 Antibody (PCRP-ZNF704-3C10) [NBP3-13765]
Flow Cytometry: ZNF704 Antibody (PCRP-ZNF704-3C10) [NBP3-13765] - Flow cytometric analysis of PFA-fixed HeLa cells. ZNF704 antibody (PCRP-ZNF704-3C10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).Applications for ZNF704 Antibody (PCRP-ZNF704-3C10)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Immunoprecipitation
1-2 ug/100-500 ug protein
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: ZNF704
Alternate Names
FLJ16218, MGC10858, zinc finger protein 704
Gene Symbol
ZNF704
UniProt
Additional ZNF704 Products
Product Documents for ZNF704 Antibody (PCRP-ZNF704-3C10)
Product Specific Notices for ZNF704 Antibody (PCRP-ZNF704-3C10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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