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ZNF704 Antibody (PCRP-ZNF704-3C10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13765

Novus Biologicals, part of Bio-Techne
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NBP3-13765-100ug
NBP3-13765-20ug

Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2C Clone # PCRP-ZNF704-3C10

Concentration

0.2 mg/ml

Product Summary for ZNF704 Antibody (PCRP-ZNF704-3C10)

Immunogen

Recombinant full-length human ZNF704 protein (Uniprot: Q6ZNC4)

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2C

Theoretical MW

45.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14168)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for ZNF704 Antibody (PCRP-ZNF704-3C10)

Immunocytochemistry/ Immunofluorescence: ZNF704 Antibody (PCRP-ZNF704-3C10) [NBP3-13765]

Immunocytochemistry/ Immunofluorescence: ZNF704 Antibody (PCRP-ZNF704-3C10) [NBP3-13765]

Immunocytochemistry/Immunofluorescence: ZNF704 Antibody (PCRP-ZNF704-3C10) [NBP3-13765] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using ZNF704 Antibody (PCRP-ZNF704-3C10) followed by goat anti-mouse IgG-CF488 (green). CF640 phalloidin (red).
Immunohistochemistry-Paraffin: ZNF704 Antibody (PCRP-ZNF704-3C10) [NBP3-13765]

Immunohistochemistry-Paraffin: ZNF704 Antibody (PCRP-ZNF704-3C10) [NBP3-13765]

Immunohistochemistry-Paraffin: ZNF704 Antibody (PCRP-ZNF704-3C10) [NBP3-13765] - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with ZNF704 antibody (PCRP-ZNF704-3C10). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Flow Cytometry: ZNF704 Antibody (PCRP-ZNF704-3C10) [NBP3-13765]

Flow Cytometry: ZNF704 Antibody (PCRP-ZNF704-3C10) [NBP3-13765]

Flow Cytometry: ZNF704 Antibody (PCRP-ZNF704-3C10) [NBP3-13765] - Flow cytometric analysis of PFA-fixed HeLa cells. ZNF704 antibody (PCRP-ZNF704-3C10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Applications for ZNF704 Antibody (PCRP-ZNF704-3C10)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Immunoprecipitation

1-2 ug/100-500 ug protein
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: ZNF704

Alternate Names

FLJ16218, MGC10858, zinc finger protein 704

Gene Symbol

ZNF704

UniProt

Additional ZNF704 Products

Product Documents for ZNF704 Antibody (PCRP-ZNF704-3C10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ZNF704 Antibody (PCRP-ZNF704-3C10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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