Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all ZZEF1 Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 525 to 575 of human Zinc Finger, ZZ-Type with EF-Hand Domain 1 using the numbering given in entry NP_055928.3 (GeneID 23140).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZZEF1 Antibody - BSA Free

Immunoprecipitation: ZZEF1 Antibody [NBP2-32119]

Immunoprecipitation: ZZEF1 Antibody [NBP2-32119]

Immunoprecipitation: ZZEF1 Antibody [NBP2-32119] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-ZZEF1 antibody NBP2-32119 used for IP at 6 ug/mg lysate. ZZEF1 was also immunoprecipitated by rabbit anti-ZZEF1 antibody NBP2-32119. For blotting immunoprecipitated ZZEF1, NBP2-32119 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for ZZEF1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

Negative

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ZZEF1

Chimera RNA interference (chimera RNAi) is process by which small interfering RNA/DNA chimera triggers the destruction of mRNA for the original gene. The discovery work, design, and application of chimera RNAi has been pioneered by Professor Kaoru Saigo and Dr. Kumiko Ui-Tei at the University of Tokyo. Chimera RNAi has many advantages over the conventional siRNAs. First, it has been demonstrated to have reliable knock-down for over 10,000 human genes. Because the human genome is composed of an intricate, genetic network, chimera RNAi's unique design has successfully obviated the off-target effects including microRNA-based influence. Another advantage of the chimera RNAi technology is its effectiveness at low concentrations (0.5nM to 5nM); only mRNA is destroyed so genomic genes are not affected. Finally, having both the sense and anti-sense strands consisting RNA/DNA chimera, it offers much greater compound stability for streamlining in vitro and in vivo assays and applications while minimizing interferon induction and other adverse reactions.

Alternate Names

FLJ10821, FLJ23789, KIAA0399, MGC166929, zinc finger, ZZ-type with EF hand domain 1, zinc finger, ZZ-type with EF-hand domain 1, ZZZ4

Gene Symbol

ZZEF1

UniProt

NP_055928.3 (Human)

Additional ZZEF1 Products

Product Documents for ZZEF1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ZZEF1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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