CellXVivo Human NK Cell Expansion Kit

Human NK Cell Expansion Using the CellXVivo™ Kit is Equivalent to Standard Feeder Cell Expansion.  Human NK cells were enriched from peripheral blood mononuclear cells and expanded (3 x 10⁶starting population) using the CellXVivo^™NK Cell Expansion Kit or the standard method using artificial antigen-presenting K562 clone 9.mbIL-21 cells. NK cell expansion was quantified at days 1, 7, 10, and 14. The rate of NK cell expansion observed using the CellXVivo^™Kit was similar to that observed using K562 cells.Data courtesy of the laboratory of Dr. Bruce Walcheck at the University of Minnesota.

Kit-expanded NK Cells are Cytotoxic to Tumor Cells. NK Cells expanded using the CellXVivo^™NK Cell Expansion Kit were assessed for toxicity against the NK cell-sensitive K562 tumor cell line. K562 cell were loaded with the live-cell dye, Calcein-AM (Tocris, Catalog # 5119) prior to being mixed with NK cells for 4 hours at the indicated effector-to-target cell (E:T) ratios (A).

Flow Cytometry Analysis of Expanded Human NK Cells.  Human PBMCs were culturedin vitrofor 14 days to expand Natural Killer cells using reag ents included in the CellXVivo^™Human NK Cell Expansion Kit. Compared to unexpanded PBMCs (A), PBMCs treated with the kit (B) show an increased number of CD3^-CD56⁺NK cells. Flow cytometric analysis was performed on day 14 of the expansion and cells were stained with Human NCAM-1/CD56 PE-conjugated Antibody, and Human CD3ε PerCP-conjugated Antibody. Quadrants were set based on isotope-stained samples.

Human NK Cells Expanded using the CellXVivo™ Kit Express NK Cell-specific Markers.  Human NK cells were enriched from peripheral blood mononuclear cells and expanded (3 x 10⁶starting population) using the CellXVivo^™NK Cell Expansion Kit (A, C, E) or by the standard method using artificial antigen-presenting K562 clone 9.mbIL-21 cells (B, D, F). After 14 days of expansion, NK cells were assessed for expression of characteristic NK cell surface markers, CD56 (A, B), NKG2D (C, D), and NKp46 (E, F). Data courtesy of the laboratory of Dr. Bruce Walcheck at the University of Minnesota.

Human NK Cells Expanded using the CellXVivo™ Kit Express High Levels of CD16. Human NK cells were enriched from peripheral blood mononuclear cells and expanded ( 3 x 10⁶starting population) using the CellXVivo^™NK Cell Expansion Kit or by the standard method using artificial antigen-presenting K562 clone 9.mbIL-21 cells. After 14 days of expansion, NK cells were assessed for CD16 expression. NK cell expanded using the CellXVivo^™Kit expressed higher levels of CD16 based on mean fluorescence intensity when compared to NK cells expanded using the K562 method.Data courtesy of the laboratory of Dr. Bruce Walcheck at the University of Minnesota.