CryoDefend-Cell Lines (5 x 10 mL)
R&D Systems, part of Bio-Techne | Catalog # CCM019
Key Product Details
|
Cell Viability Following Cryopreservation in CryoDefend®Cell Lines Media in Comparison to Conventional Cryopreservation Media. A variety of cell lines were frozen in triplicate in either control freezing media (90% culture media/10% DMSO) or CryoDefend®Cell Lines (CryoDefend®-CL) media at 1 x 106cells/cryovial and stored in liquid nitrogen for one week. The cells were then thawed, counted (blue bars), resuspended in standard culture media, and plated into T75 flasks. After 3-5 days in culture, the cells were harvested and counted (red bars). Error bars shown indicate the standard deviation. |
Assay Procedure
Refer to the product datasheet for complete product details.
Briefly, cultured cells can be cryopreserved using the following procedure:
- Transfer detached cells to a conical tube and centrifuge
- Remove supernatant and resuspend in CryoDefend® Cell Lines Media
- Transfer cells to a cryovial and freeze at -80 °C overnight
- Transfer the frozen cryovial to liquid nitrogen
Reagents supplied in the CryoDefend® Cell Lines Media (Catalog # CCM019):
- 5 x 10 mL vials of CryoDefend® Cell Lines Media
Upon receipt, the CryoDefend® Cell Lines Media should be stored at = -20 °C in a manual defrost freezer. The media can be thawed at 2 °C to 8 °C or at room temperature. Thawed media can be aliquoted and stored at = -20 °C in a manual defrost freezer for up to 3 months. Thaw a fresh aliquot for each use. Avoid repeated freeze-thaw cycles.
Reagents
- Cell culture media
Materials
- Cultured cells
- Cryovials
- 15 mL centrifuge tubes
- Serological pipettes
- Pipette and pipette tips
Equipment
- 37 °C and 5% CO2 incubator
- Centrifuge
R&D Systems Protocol for the Cryopreservation and Thawing of Stem Cells in CryoDefend® Cell Lines Media (Catalog # CCM019)
Cryopreservation of Cultured Cells
Thaw CryoDefend® Cell Lines Media.
Detach the cells from the cell culture dish.
Transfer the cells to a 15 mL conical tube.
Centrifuge at 200 x g for 5 minutes.
Remove and discard the supernatant.
Resuspendthe cells in CryoDefend® Cell Lines Media at 0.5-1.0 x 106 cells/mL.
Transfer the cells to a cryovial.
Freeze the cryovial at -80 °C overnight.
Transfer the frozen cryovial to liquid nitrogen for storage.
Thawing of Cryopreserved Cells
Warm appropriate cell culture media to 37 °C.
Add pre-warmed culture media to the cryovial containing cryopreserved cells.
Pipette up and down and as cells thaw, transfer the thawed cells to a 15 mL conical tube containing pre-warmed expansion media.
Centrifuge at 200 x g for 5 minutes.
Resuspend the cells in pre-warmed expansion media.
Plate the stem cells at the desired density.
Customer Reviews for CryoDefend-Cell Lines (5 x 10 mL)
There are currently no reviews for this product. Be the first to review CryoDefend-Cell Lines (5 x 10 mL) and earn rewards!
Have you used CryoDefend-Cell Lines (5 x 10 mL)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review