What type of analysis is typically applied for organoid or 3-D cell cultures?

Within the organoid, spheroid, or 3-D culture, cells may be assessed for morphology, apical/basal polarity, protein localization, and relative proliferation. In addition, cells may be isolated from the 3-D culture and evaluated for levels of RNA and protein expression, as well as modifications to DNA.

What is the difference between the standard Cultrex Rat Collagen I and the Cultrex 3-D Rat Collagen I?

Cultrex 3-D Rat Collagen I undergoes the same basic purification and efficacy tests as the standard collagen. However, it undergoes additional 3-D culture validation; it has been tested extensively for the ability to promote growth and differentiation of cell types, visualized by morphology in three dimensions in vitro.

What are 3-D cultures?

3-D cultures are in vitro cultures where immortalized cell lines, primary cell lines, stem cells, or tissue explants are placed within hydrogel matrices, such as Cultrex Basement Membrane Extract, that mimic in vivo cell environments and allow cells to proliferate in three dimensions.

What is the advantage of 3-D culture over traditional 2-D culture?

While 2-D culture has been used for studying many aspects of cell function and behavior, the tissue-culture treated plastic environment is unlike anything found within living organisms. As a result, cells in 2-D culture exhibit altered morphology, function, proliferation, and gene expression when compared to their emanating tissues. By placing these cells in a 3-D environment, they assume biological and biochemical characteristics similar to what is observed in vivo.

What are the variables associated with 3-D culture?

The major variables associated with 3-D culture are cell type, cell seeding density, composition of hydrogel, thickness of hydrogel, stiffness of hydrogel, composition of cell culture medium, and time of culture.

What are the different types of 3-D culture?

The two principal methods for performing 3-D culture are the top assay and embedded assay. For the top assay, cells are seeded on a thick gel of Cultrex Basement Membrane Extract (BME) or Extracellular Matrix Protein. A thin overlay of cell culture medium is then applied to the cells. For the embedded assay, cells are resuspended within a thick gel of Cultrex BME or ECM and the culture media is applied on top. The top assay is easier to setup, to control seeding densities, and to keep cells within one focal plane for analysis.

How should cells be cultured prior to setting up the 3-D culture?

Cells need to be healthy and actively dividing in 2-D culture. Cells should be passaged two or three times after resuspension from cryopreservation, and they should never surpass 80% confluency during each passage. Cells should also be assessed for viability using trypan blue, and they should exhibit less than 5% staining.

Will Cultrex 3-D culture Matrix rat Collagen 1, Catalog # 3447-020-01, still work if it has been frozen?

This product will likely not form a gel after being frozen.

Is Cultrex 3-D Culture Matrix Rat Collagen I (Catalog # 3447-020-01) atelocollagen or telocollagen?

Cultrex 3-D Culture Matrix Rat Collagen I (Catalog # 3447-020-01) is telocollagen.

Can the Organoid Harvesting Solution, Catalog # 3700-100-01, be used to dissociate cells grown on a substrate of Cultrex Rat Collagen 1?

The Organoid Harvesting Solution works well with BME (Basement Membrane Extract). For cells grown on Cultrex Rat Collagen 1, the recommended enzyme to use to dissociate cells is Collagenase. Collagenase treatment at 37oC for 30 minutes is recommended.

Does the stability of Cultrex 3-D Culture Matrix Rat Collagen I (Catalog # 3447-020-01) change after opening?

We recommend storing the product at 2-8 °C, and provided it has not been neutralized with NaOH or Sodium Bicarbonate, it should remain stable and perform as expected as long as it is used prior to the expiration date.

Cultrex 3-D Culture Matrix Rat Collagen I Best Seller

Name: Cultrex 3-D Culture Matrix Rat Collagen I
Brand: R&D Systems
SKU: 3447-020-01
Rating: 3 (1 reviews)

R&D Systems, part of Bio-Techne | Catalog # 3447-020-01

(1)

Citations (48)

Product Datasheet / COA / SDS

Rat Collagen I specifically qualified to support 3D culture applications.

Key Product Details

Features:

3-D Culture Matrix Collagen I is an extracellular matrix hydrogel that directs cells to grow in three dimensions and assemble into organotypic structures in vitro.

Technical Data
Citations / Reviews (1) / FAQs

Summary for Cultrex 3-D Culture Matrix Rat Collagen I

Why Use Cultrex 3-D Culture Matrix Rat Collagen I?

Cultrex 3-D Culture Matrix Collagen I is purified from rat tail tendons and is provided at a high concentration that is capable of polymerizing to form a hydrogel. Collagen I is the major structural component of extracellular matrices (ECM) found in connective tissue and internal organs, but is most prevalent in the dermis, tendons, and bone. It is a 300 kDa molecule composed of two alpha1(I) chains and one alpha2(I) chain that spontaneously forms a triple helix scaffold at a neutral pH and 37̊ C. This phenomenon can be exploited to promote cell attachment, growth, differentiation, migration, and tissue morphogenesis during development. To provide the most standardized Collagen I for use in 3-D cultures, a special process is employed to provide material at a standard concentration of approximately 5 mg/mL. This material is then incorporated in a 3-D culture to validate efficacy.Cultrex 3-D Culture Matrix Collagen I is a purified stromal ECM protein that has been developed, produced and qualified specifically for use in 3-D culture studies. 3-D Culture Matrix Collagen I may also be used to supplement customized hydrogel or medium formulations for cell culture.

Negative for the presence of bacteria and fungi.

Product Specifications for Cultrex 3-D Culture Matrix Rat Collagen I

Species	Rat
Source	Rat tail tendons
Protein Concentration	5 mg/mL
Endotoxin level	≤ 20 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing	No bacterial or fungal growth detected following 14 days in culture
Cell Culture Testing	3-D Culture - Laminin I Supports differentiation of a human epithelial cell line derived from mammary gland (MCF-10A) into acinar structures. Cell Attachment - Tested for the ability to Supports cell attachment and spreading of MG63 human osteosarcoma cells.
Viral Testing	Negative by PCR test for mycoplasma; 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, plus 13 additional murine infectious agents including LDEV, for a total of 31 organisms and viruses.

Preparation & Storage

Stability	Product is stable for a minimum of 2 months from date of receipt if stored at 2-8 °C. See lot specific Certificate of Analysis for expiration date.
Shipping Conditions	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage	Store the unopened product at 2 - 8 °C.

Cultrex 3-D Culture Matrix Rat Collagen I Best Seller

R&D Systems, part of Bio-Techne | Catalog # 3447-020-01

Key Product Details

Summary for Cultrex 3-D Culture Matrix Rat Collagen I

Why Use Cultrex 3-D Culture Matrix Rat Collagen I?

Product Specifications for Cultrex 3-D Culture Matrix Rat Collagen I

Preparation & Storage

FAQs for Cultrex 3-D Culture Matrix Rat Collagen I

Product Documents for Cultrex 3-D Culture Matrix Rat Collagen I

Product Datasheets

COA

Certificate of Analysis

SDS

CONTACT INFORMATION

Stay Connected