What is the recommended working concentration for Cultrex Laminin I?

The recommended working concentration for thin coating is 0.05-10 µg/cm2 . However, conditions must be optimized for each cell line or model. Cultrex Laminin I should be used at 6 mg/mL for 3-D culture applications.

Can Cultrex Mouse Laminin I be used on glass cover slips?

Yes. Coating glass cover slips with Cultrex Laminin I is quite common and has been published extensively.

Cultrex Antibiotic-free Mouse Laminin I, Pathclear

Name: Cultrex Antibiotic-free Mouse Laminin I, Pathclear
Brand: R&D Systems
SKU: 3401-010-02

R&D Systems, part of Bio-Techne | Catalog # 3401-010-02

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Citations (3)

Product Datasheet / COA / SDS

Mouse Laminin I purified and qualified for use in antibiotic-free cell culture.

Key Product Details

Features:	Cultrex Antibiotic-Free Mouse Laminin I is a purified basement membrane protein that has been developed and qualified as a extracellular matrix coating solution for cell attachment to tissue culture vessels.
Key Benefits:	Produced and qualified for use in cell culture Antibiotic-free formulation Pathclear-tested to ensure a pathogen and virus-free material Source tissue is LDEV-free

Technical Data
Citations / Reviews / FAQs

Summary for Cultrex Antibiotic-free Mouse Laminin I, Pathclear

Why Use Cultrex Antibiotic-free Mouse Laminin I?

Cultrex Antibiotic-Free Mouse Laminin I is purified from Engelbreth-Holm-Swarm (EHS) tumor and is provided in antibiotic free medium. Laminin I is a major component of the basement membrane which is a continuous sheet of specialized extracellular matrix that forms an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that plays an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation.

Negative for the presence of bacteria and fungi.

Product Specifications for Cultrex Antibiotic-free Mouse Laminin I, Pathclear

Species	Mouse
Source	Murine Engelbreth-Holm-Swarm (EHS) tumor
Protein Concentration	1 mg/mL
Endotoxin level	≤ 20 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing	No bacterial or fungal growth detected following 14 days in culture
Cell Culture Testing	Supports cell attachment and spreading of MG63 human osteosarcoma cells.
Viral Testing	Tested negative by PCR test for a total of 31 organisms and viruses, including: mycoplasma, 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, and 13 additional murine infectious agents including LDEV.

Preparation & Storage

Stability	Product is stable for a minimum of 3 months from date of shipment. See lot specific Certificate of Analysis for expiration date.
Shipping Conditions	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage	Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

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Cultrex Antibiotic-free Mouse Laminin I, Pathclear

R&D Systems, part of Bio-Techne | Catalog # 3401-010-02

Key Product Details

Summary for Cultrex Antibiotic-free Mouse Laminin I, Pathclear

Why Use Cultrex Antibiotic-free Mouse Laminin I?

Product Specifications for Cultrex Antibiotic-free Mouse Laminin I, Pathclear

Preparation & Storage

Customer Reviews for Cultrex Antibiotic-free Mouse Laminin I, Pathclear

FAQs for Cultrex Antibiotic-free Mouse Laminin I, Pathclear

Product Documents for Cultrex Antibiotic-free Mouse Laminin I, Pathclear

Product Datasheets

COA

Certificate of Analysis

SDS

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