What kinds of tumor cells or biopsy specimens grow in vivo with Cultrex® BME?

Many cell lines and tumor biopsy specimens (usually cut into small fragments) have been found to grow in vivo when implanted with Cultrex® BME. These include melanoma, intestinal, prostate, breast, lung, renal, and liver cancers as well as the 3T3 mouse embryonic fibroblast cell line.

How does Cultrex® Basement Membrane Extract (BME) promote cell differentiation?

All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic surfaces, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.

How should Cultrex Basement Membrane Extract (BME) be stored and handled?

Cultrex BME should be stored at or below -20°C for optimal stability. Preparation of working aliquots is recommended. Cultrex BME should be thawed overnight on ice at 4°C, however long term storage at 4°C is not recommended. Freeze/thaw cycles and gel-liquid phase transitions should be avoided, since they can compromise product integrity.

What is the percent of Laminin I in Cultrex® Basement Membrane Extract, PathClear, Catalog # 3432-010-01?

Cultrex® Basement Membrane Extract (BME), Pathclear, is composed of ≤ 60 % of Laminin I. Laminin is the major component of Cultrex BME. Other components are Collagen IV, which makes up ≤ 40% and Entactin which makes up ≤ 10%.

Cultrex Basement Membrane Extract, PathClear, with Phenol Red Best Seller

Name: Cultrex Basement Membrane Extract, PathClear, with Phenol Red
Brand: R&D Systems
SKU: 3432-010-01P

R&D Systems, part of Bio-Techne | Catalog # 3432-010-01P

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Product Datasheet / COA / SDS

Basement Membrane Extract for use in multiple culture applications

Key Product Details

Features:	Cultrex Basement Membrane Extract (BME) is an extracellular matrix hydrogel that has been used extensively in general cell culture applications to study cell attachment, proliferation, and invasion.
Key Benefits:	Ideal for general cell culture application Polymerizes at 37°C to form a reconstituted basement membrane Quality controlled for peformance consistency

Technical Data
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Summary for Cultrex Basement Membrane Extract, PathClear, with Phenol Red

Why Use Cultrex BME?

Cultrex Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Cultrex BME can be used in multiple applications, including maintaining growth or promoting differentiation of primary endothelial, epithelial, smooth muscle, stem cells, and organoid/3-D cell cultures. It can also be utilized in cell attachment, neurite outgrowth, angiogenesis, in vitro cell invasion, and in vivo tumorigenicity assays.

Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparin sulfate proteoglycans.

Kit Contents for Cultrex Basement Membrane Extract, PathClear, with Phenol Red

Cultrex Basement Membrane Extract
Phenol Red

Product Specifications for Cultrex Basement Membrane Extract, PathClear, with Phenol Red

Species	Mouse
Source	Murine Engelbreth-Holm-Swarm (EHS) tumor
Protein Concentration	8-12 mg/mL as determined by Lowry assay
Endotoxin level	≤ 8 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing	No bacterial or fungal growth detected following 14 days in culture
Cell Culture Testing	Tube Formation Assay - Cultrex BME supports formation of capillary-like structures by human (HBMVEC; HUVEC) or mouse (SVEC4-10) endothelial cells in a tube formation assay.
Viral Testing	Tested negative by PCR test for a total of 31 organisms and viruses, including: mycoplasma, 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, and 13 additional murine infectious agents including LDEV.

Preparation & Storage

Stability	Product is stable for at least two years from date of manufacture when stored at ≤ -70 °C. See lot specific Certificate of Analysis for expiration date.
Shipping Conditions	The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

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Cultrex Basement Membrane Extract, PathClear, with Phenol Red Best Seller

R&D Systems, part of Bio-Techne | Catalog # 3432-010-01P

Key Product Details

Summary for Cultrex Basement Membrane Extract, PathClear, with Phenol Red

Why Use Cultrex BME?

Kit Contents for Cultrex Basement Membrane Extract, PathClear, with Phenol Red

Product Specifications for Cultrex Basement Membrane Extract, PathClear, with Phenol Red

Preparation & Storage

Customer Reviews for Cultrex Basement Membrane Extract, PathClear, with Phenol Red

FAQs for Cultrex Basement Membrane Extract, PathClear, with Phenol Red

Product Documents for Cultrex Basement Membrane Extract, PathClear, with Phenol Red

Product Datasheets

COA

Certificate of Analysis

SDS

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