What kinds of tumor cells or biopsy specimens grow in vivo with Cultrex® BME?

Many cell lines and tumor biopsy specimens (usually cut into small fragments) have been found to grow in vivo when implanted with Cultrex® BME. These include melanoma, intestinal, prostate, breast, lung, renal, and liver cancers as well as the 3T3 mouse embryonic fibroblast cell line.

How should Cultrex Basement Membrane Extract (BME) be stored and handled?

Cultrex BME should be stored at or below -20°C for optimal stability. Preparation of working aliquots is recommended. Cultrex BME should be thawed overnight on ice at 4°C, however long term storage at 4°C is not recommended. Freeze/thaw cycles and gel-liquid phase transitions should be avoided, since they can compromise product integrity.

What type of culture plates are recommended for organoid culture and other cultures using Cultrex BME products?

Any plate that is tissue culture treated will bind Cultrex domes/Cultrex coatings. Non-treated or low attachment plates are not recommended for organoid growth and for other cell cultures using coated BME. The domes will float away if non-treated or low-attachment plates are used. Bio-Techne has used the folllowing plates: Corning 96-well, Catalog # 3595; Corning 24-well, Catalog #3526; ThermoFisher 6-well, Catalog # 140675.

Cultrex Basement Membrane Extract, Type 2, Pathclear, with Phenol Red

Name: Cultrex Basement Membrane Extract, Type 2, Pathclear, with Phenol Red
Brand: R&D Systems
SKU: 3532-010-02P

R&D Systems, part of Bio-Techne | Catalog # 3532-010-02P

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Product Datasheet / COA / SDS

For Robust Organoid Cultures - Basement Membrane Extract

Key Product Details

Features:

Cultrex Basement Membrane Extract (BME), Type 2 is specifically designed to support the establishment and expansion of robust organoid cultures. It's composition mimics the in vivo microenvironment to improve take rate and growth of organoid progenitor cells.

Technical Data
Citations / Reviews / FAQs

Summary for Cultrex Basement Membrane Extract, Type 2, Pathclear, with Phenol Red

Why Use Cultrex BME, Type 2?

Cultrex Basement Membrane Extract (BME), Type 2 is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparan sulfate proteoglycans.

Cultrex RGF BME, Type 2 provides a proprietary formulation that has a high storage modulus and is designed for use in robust tissue organoid culture as well as other applications requiring an extracellular matrix scaffold.

Kit Contents for Cultrex Basement Membrane Extract, Type 2, Pathclear, with Phenol Red

Cultrex Basement Membrane Extract (BME), Type 2
Phenol Red

Product Specifications for Cultrex Basement Membrane Extract, Type 2, Pathclear, with Phenol Red

Species	Mouse
Source	Murine Engelbreth-Holm-Swarm (EHS) tumor
Protein Concentration	8-12 mg/mL as determined by Lowry assay
Endotoxin level	≤ 8 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing	No bacterial or fungal growth detected following 14 days in culture
Cell Culture Testing	Organoid Culture - Cultrex BME, Type 2 supports growth and expansion of mouse small intestine organoid progenitor cells. Gelling Assay - Cultrex BME, Type 2 gels in less than 30 minutes at 37 °C, and maintains the gelled form in culture medium for a minimum of 7 days at 37 °C. Dome Assay Cultrex BME, Type 2 forms and maintains stable 3-D dome structures on cell culture plates. Tube Formation Assay - Cultrex BME, Type 2 supports formation of capillary-like structures by human (HBMVEC; HUVEC) or mouse (SVEC4-10) endothelial cells.
Viral Testing	Tested negative by PCR test for a total of 31 organisms and viruses, including: mycoplasma, 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, and 13 additional murine infectious agents including LDEV.

Preparation & Storage

Stability	Product is stable for at least two years from date of manufacture when stored at ≤ -70 °C. See lot specific Certificate of Analysis for expiration date.
Shipping Conditions	The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

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Cultrex Basement Membrane Extract, Type 2, Pathclear, with Phenol Red

R&D Systems, part of Bio-Techne | Catalog # 3532-010-02P

Key Product Details

Summary for Cultrex Basement Membrane Extract, Type 2, Pathclear, with Phenol Red

Why Use Cultrex BME, Type 2?

Kit Contents for Cultrex Basement Membrane Extract, Type 2, Pathclear, with Phenol Red

Product Specifications for Cultrex Basement Membrane Extract, Type 2, Pathclear, with Phenol Red

Preparation & Storage

Customer Reviews for Cultrex Basement Membrane Extract, Type 2, Pathclear, with Phenol Red

FAQs for Cultrex Basement Membrane Extract, Type 2, Pathclear, with Phenol Red

Product Documents for Cultrex Basement Membrane Extract, Type 2, Pathclear, with Phenol Red

Product Datasheets

COA

Certificate of Analysis

SDS

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