What kinds of tumor cells or biopsy specimens grow in vivo with Cultrex® BME?

Many cell lines and tumor biopsy specimens (usually cut into small fragments) have been found to grow in vivo when implanted with Cultrex® BME. These include melanoma, intestinal, prostate, breast, lung, renal, and liver cancers as well as the 3T3 mouse embryonic fibroblast cell line.

How does Cultrex® Basement Membrane Extract (BME) promote cell differentiation?

All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic surfaces, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.

How should Cultrex Basement Membrane Extract (BME) be stored and handled?

For best practices for handling our BME products, please refer to our helpful guide at the following link: https://www.bio-techne.com/reagents/cell-culture-reagents/cultrex-bme-and-ecm-proteins/cell-culture-matrix-for-your-research

What type of culture plates are recommended for organoid culture and other cultures using Cultrex BME products?

Any plate that is tissue culture treated will bind Cultrex domes/Cultrex coatings. Non-treated or low attachment plates are not recommended for organoid growth and for other cell cultures using coated BME. The domes will float away if non-treated or low-attachment plates are used. Bio-Techne has used the folllowing plates: Corning 96-well, Catalog # 3595; Corning 24-well, Catalog #3526; ThermoFisher 6-well, Catalog # 140675.

Cultrex Basement Membrane Extract, Type 2, Pathclear Best Seller

Name: Cultrex Basement Membrane Extract, Type 2, Pathclear
Brand: R&D Systems
SKU: 3532-010-02
Rating: 5 (1 reviews)

R&D Systems, part of Bio-Techne | Catalog # 3532-010-02

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Product Datasheet / COA

For Robust Organoid Cultures - Basement Membrane Extract

Try it on your cultures! Request a Sample of Cultrex BME, Type 2

Key Product Details

Features:	Cultrex Basement Membrane Extract (BME), Type 2 is specifically designed to support the establishment and expansion of robust organoid cultures. It's composition mimics the in vivo microenvironment to improve take rate and growth of organoid progenitor cells.
Key Benefits:	Qualified for use in organoid cell culture Commonly used robust and established organoid systems Quality controlled for peformance consistency

Technical Data
Citations / Reviews (1) / FAQs

Summary for Cultrex Basement Membrane Extract, Type 2, Pathclear

Why Use Cultrex BME, Type 2?

Cultrex Basement Membrane Extract (BME), Type 2 is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparan sulfate proteoglycans.

Cultrex RGF BME, Type 2 provides a proprietary formulation that has a high storage modulus and is designed for use in robust tissue organoid culture as well as other applications requiring an extracellular matrix scaffold.

Product Specifications for Cultrex Basement Membrane Extract, Type 2, Pathclear

Species	Mouse
Source	Murine Engelbreth-Holm-Swarm (EHS) tumor
Protein Concentration	8-12 mg/mL as determined by Lowry assay
Endotoxin level	≤ 8 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing	No bacterial or fungal growth detected following 14 days in culture
Cell Culture Testing	Organoid Culture - Cultrex BME, Type 2 supports growth and expansion of mouse small intestine organoid progenitor cells. Gelling Assay - Cultrex BME, Type 2 gels in less than 30 minutes at 37 °C, and maintains the gelled form in culture medium for a minimum of 7 days at 37 °C. Dome Assay Cultrex BME, Type 2 forms and maintains stable 3-D dome structures on cell culture plates. Tube Formation Assay - Cultrex BME, Type 2 supports formation of capillary-like structures by human (HBMVEC; HUVEC) or mouse (SVEC4-10) endothelial cells.
Viral Testing	Tested negative by PCR test for a total of 31 organisms and viruses, including: mycoplasma, 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, and 13 additional murine infectious agents including LDEV.

Preparation & Storage

Stability	Product is stable for at least two years from date of manufacture when stored at ≤ -70 °C. See lot specific Certificate of Analysis for expiration date.
Shipping Conditions	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage	Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Cultrex Basement Membrane Extract, Type 2, Pathclear Best Seller

R&D Systems, part of Bio-Techne | Catalog # 3532-010-02

Key Product Details

Summary for Cultrex Basement Membrane Extract, Type 2, Pathclear

Why Use Cultrex BME, Type 2?

Product Specifications for Cultrex Basement Membrane Extract, Type 2, Pathclear

Preparation & Storage

FAQs for Cultrex Basement Membrane Extract, Type 2, Pathclear

Product Documents for Cultrex Basement Membrane Extract, Type 2, Pathclear

Product Datasheets

COA

Certificate of Analysis

CONTACT INFORMATION

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