What kinds of tumor cells or biopsy specimens grow in vivo with Cultrex® BME?

Many cell lines and tumor biopsy specimens (usually cut into small fragments) have been found to grow in vivo when implanted with Cultrex® BME. These include melanoma, intestinal, prostate, breast, lung, renal, and liver cancers as well as the 3T3 mouse embryonic fibroblast cell line.

How should Cultrex Basement Membrane Extract (BME) be stored and handled?

Cultrex BME should be stored at or below -20°C for optimal stability. Preparation of working aliquots is recommended. Cultrex BME should be thawed overnight on ice at 4°C, however long term storage at 4°C is not recommended. Freeze/thaw cycles and gel-liquid phase transitions should be avoided, since they can compromise product integrity.

Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2, Pathclear, with Phenol Red

Name: Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2, Pathclear, with Phenol Red
Brand: R&D Systems
SKU: 3533-010-02P
Rating: 5 (1 reviews)

R&D Systems, part of Bio-Techne | Catalog # 3533-010-02P

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Citations (1)

Product Datasheet / COA / SDS

For Robust Organoid Cultures - Reduced Growth Factor Basement Membrane Extract

Key Product Details

Features:	Cultrex Reduced Growth Factor Basement Membrane Extract (RGF BME ),Type 2 is specifically qualified to support the establishment and expansion of robust organoid cultures. It's composition mimics the in vivo microenvironment to improve take rate and growth of organoids.
Key Benefits:	Qualified for use in organoid cell culture Commonly used robust and established organoid systems Reduced growth factor formulation provides a more defined culture system Quality controlled for performance consistency

Technical Data
Citations / Reviews (1) / FAQs

Summary

Why Use Cultrex RGF BME, Type 2?

Cultrex Reduced Growth Factor Basement Membrane Extract (RGF BME), Type 2 is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparan sulfate proteoglycans.

Cultrex RGF BME, Type 2 provides a proprietary formulation that is high in tensile strength and is designed for use in robust tissue organoid culture as well as other applications requiring an extracellular matrix scaffold.

Protocols Utilizing Cultrex RGF BME, Type 2 for Organoid Cell Culture.

Cultrex RGF BME, Type 2 is ideal for use as a scaffold for organoid and 3D cell culture. Listed below are protocols designed by our research and development groups for organoid culture featuring Cultrex RGF BME, Type 2 as well as growth factors, media supplements, and small molecules from Bio-Techne.
Video of Cultrex BME Best Practices and Protocols.
Protocol for Mouse Enteric Organoid Culture.
Protocol for Human Gastric Organoid Culture.
Protocol for Human Liver Organoid Culture.
Protocol for Human Lung Organoid Culture.
Protocol for Human Intestinal Organoid Culture.
Protocol for Harvesting Organoids for Biochemical Analysis.

Kit Contents

Cultrex Reduced Growth Factor Basement Membrane Extract (RGF BME), Type 2
Phenol Red

Product Specifications

Species	Mouse
Source	Murine Engelbreth-Holm-Swarm (EHS) tumor
Protein Concentration	8-12 mg/mL as determined by Lowry assay
Endotoxin level	≤ 8 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing	No bacterial or fungal growth detected following 14 days in culture
Cell Culture Testing	Organoid Culture - Cultrex RGF BME, Type 2 supports growth and expansion of mouse small intestine organoid progenitor cells. Gelling Assay - Cultrex RGF BME, Type 2 gels in less than 30 minutes at 37 °C, and maintains the gelled form in culture medium for a minimum of 7 days at 37 °C. Dome Assay Cultrex RGF BME, Type 2 forms and maintains stable 3-D dome structures on cell culture plates. Tube Formation Assay - Cultrex RGF BME, Type 2 supports formation of capillary-like structures by human (HBMVEC; HUVEC) or mouse (SVEC4-10) endothelial cells.
Viral Testing	Tested negative by PCR test for a total of 31 organisms and viruses, including: mycoplasma, 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, and 13 additional murine infectious agents including LDEV.

Preparation & Storage

Stability	Product is stable for at least two years from date of manufacture when stored at ≤ -70 °C. See lot specific Certificate of Analysis for expiration date.
Shipping Conditions	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2, Pathclear, with Phenol Red

R&D Systems, part of Bio-Techne | Catalog # 3533-010-02P

Key Product Details

Summary

Why Use Cultrex RGF BME, Type 2?

Protocols Utilizing Cultrex RGF BME, Type 2 for Organoid Cell Culture.

Kit Contents

Product Specifications

Preparation & Storage

FAQs

Product Documents

Product Datasheets

COA

Certificate of Analysis

SDS

CONTACT INFORMATION

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