ExCellerate™ Human NK Cell Expansion Media, Animal Component-Free

Only ExCellerate CCM037 supports both feeder-free and serum-free expansion of NK cells. Purified NK cells were plated at 2.5e⁶/cm² in a 24-well G-Rex using media without serum supplementation. NK cells were stimulated with 250 IU/mL IL-2, 2 ng/mL IL-12 (at initial seeding only), and 10 ng/mL each of IL-15, IL-18, and IL-21. After 7 days, NK cells were reseeded at a density of 0.05e⁶/cm² and grown for a total of 14 days. Data labels are cell viability at harvest.

ExCellerate CCM037 supports feeder-free expansion of NK cells better than competitor medias. Purified NK cells were plated at 2.5e⁶/cm² in a 24-well G-Rex using medias supplemented with 5% hAB serum. NK cells were stimulated with 250 IU/mL IL-2, 2 ng/mL IL-12 (at initial seeding only), and 10 ng/mL each of IL-15, IL-18, and IL-21. After 7 days, NK cells were reseeded at a density of 0.05e⁶/cm² and grown for a total of 14 days. Data labels are cell viability at harvest.

NK cells grown in ExCellerate CCM037 with or without serum show similar phenotypes. Day 14 expression profiles of NK cells grown in feeder-free, cytokine stimulated culture conditions either with or without serum supplementation, 3 donor average.

NK cells grown under feeder-free conditions in serum-containing medias maintain cytotoxic activity. Day 14 cytokine-expanded NK cells were challenged with K562 target cells in a 4 hour luciferase kill assay. NK cells grown in CCM037 show cytotoxic activity comparable to competitor medias.

ExCellerate CCM037 supports serum free expansion of gene edited NK cells. Purified NK cells were seeded in a G-Rex culture vessel using CCM037 without serum supplementation and stimulated with K562 feeder cells. Gene editing was performed on day 4 using TcBuster transposase to insert a CD19-CAR. NK cells were restimulated at day 7 and 14 with K562 feeder cells. Data labels are cell viability at harvest.