HBSS, Ca and Mg salts, phenol red

Hanks’ Balanced Salt Solution (HBSS) is an isotonic buffer solution containing inorganic salts and a carbohydrate as an energy source. It is used for various cell culture applications including the short -term maintenance of cells in a non-CO₂ environment, washing cells before dissociation or counting, and transporting cells or tissues. Formulations are available with and without calcium and magnesium salts, and with and without phenol red.