ITS-Plus Media Supplement (100X)
R&D Systems, part of Bio-Techne | Catalog # AR014
Key Product Details
Assay Procedure
Refer to the product datasheet for complete product details.
Briefly, ITS Media Supplement is prepared using the following procedure:
- Dilute the media supplement in basal media
- Store completed media at 2 – 8 °C
Reagents provided in the ITS-Plus Media Supplement (Catalog # AR014):
Component | Amount (mg/mL) |
Recombinant Human Insulin | 1.0 |
Human Transferrin | 0.5 |
Selenite | 6.4 x 10-4 |
Linoleic Acid | 0.5 |
BSA | 100 |
Stability and Storage
Store in the dark at < -20 °C in a manual defrost freezer. Do not use past the expiration date.
Precautions
This product contains human transferrin. This transferrin was tested at the donor level using an FDA licensed method and found to be non-reactive for anti-HIV-1/2 and Hepatitis B surface antigen. As no testing can offer complete assurance of freedom from infectious reagents, these reagents should be handled as if capable of transmitting infection.
Materials
- Basal media
- Serological pipettes
- Pipettes and pipette tips
Equipment
- 2 °C to 8 °C refrigerator
Dilute 100-fold into cell culture media of interest before use. The final concentration of serum required with ITS supplementation is cell type and assay dependent. Titrate for individual use.
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