Mammary Epithelial Cell Growth Media

Media for the serum-free expansion of mammary epithelial cells.

Why Use Pre-optimized, Serum-free Media to Grow Endothelial Cells?

Primary mammary epithelial cells are commonly used for the study of disease mechanisms and drug discovery in breast tissue cancer/disease. Mammary epithelial cells are phenotypically characterized as Cytokeratin 14⁺, Cytokeratin 18⁺, and Cytokeratin 19^-.

R&D Systems^® Mammary Epithelial Cell Growth Media is formulated to support the expansion of human primary mammary epithelial cells under serum-free conditions. The media has been tested for its ability to support Cytokeratin 14⁺, Cytokeratin 18⁺, and Cytokeratin 19^- human mammary epithelial cell growth in vitro.

This kit contains the following reagents to maintain mouse pluripotent stem cells in culture:

• Mammary Epithelial Cell Growth Base Media (250 mL)
• Mammary Epithelial Cell Growth Supplement (50X)

Note: The components for this kit require different storage/shipping temperatures and will arrive in separate packaging.

Stability and Storage

Store in the dark at ≤ -20 °C in a manual defrost freezer. Do not use past the expiration date.

Precaution

When handling biohazardous materials such as human cells, safe laboratory procedures should be followed and protective clothing should be worn.

Limitations

• FOR LABORATORY RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
• The safety and efficacy of this product in diagnostic or other clinical uses has not been established.
• This reagent should not be used beyond the expiration date indicated on the label.
• Results may vary due to variations among primary epithelial cells originating from different donors.

Mammary Epithelial Cell Growth Media Supports Cell Proliferation of Human Mammary Epithelial Cells (HMECs). One well of a 6-well plate of HMECs (6.7 x 104) cells was expanded over 5 passages using Complete Mammary Epithelial Cell Growth Media. Cells were passaged every 3-5 days. The histogram shows the cumulative number of HMECs expanded over 5 passages if all cells were replated at each passage.

Human Mammary Epithelial Cells (HMECs) Express Mammary Epithelial Cell Markers Cytokeratin 14, Cytokeratin 18, and E-Cadherin. HMECs were expanded in Complete Mammary Epithelial Cell Growth Media for 5 passages prior to staining for marker expression by immunocytochemistry. Cells were double stained with Goat Anti-Mouse E-Cadherin (green; R&D Systems®, Catalog # AF648) and either (A) Mouse Anti-Human Cytokeratin 14 Antibody (red; R&D Systems®, Catalog # MAB3164) or (B) Mouse Anti-Human Cytokeratin 18 Antibody (red; R&D Systems®, Catalog # 5748). The nuclei were counterstained with DAPI (blue; Tocris®, Catalog # 5748).